Cuprizone Model as a Tool for Preclinical Studies of the Efficacy of Multiple Sclerosis Diagnosis and Therapy

To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing di...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2015-05, Vol.159 (1), p.111-115
Main Authors: Abakumova, T. O., Kuz’kina, A. A., Zharova, M. V., Pozdeeva, D. A., Gubskii, I. L., Shepeleva, I. I., Antonova, O. M., Nukolova, N. V., Kekelidze, Z. I., Chekhonin, V. P.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brain - diagnostic imaging
Cell Biology
Cuprizone - pharmacology
Demyelinating Diseases - chemically induced
Diagnostic imaging
Disease Models, Animal
Drug therapy
Female
Glial Fibrillary Acidic Protein
Immunohistochemistry
Intermediate filament proteins
Internal Medicine
Laboratory Medicine
Magnetic Resonance Imaging
Male
Mice
Mice, Inbred C57BL
Multiple sclerosis
Multiple Sclerosis - chemically induced
Multiple Sclerosis - therapy
Myelin Sheath - metabolism
Nerve Tissue Proteins - metabolism
Neurophysiology
Pathology
Radiography
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Summary:To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing diet. Immunohistochemical staining with polyclonal antibodies to glial fibrillary acidic protein (pAb GFAP) confirmed the increase in the number of reactive astrocytes on week 4 of diet and during remyelination (week 2 after diet). Analysis of neurophysiological functions in mice with cuprizone-induced demyelination revealed motor and behavioral deficits. This model can be used as a tool for preclinical studies of the efficiency of multiple sclerosis diagnostic and therapy.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-015-2903-z