Sequential delivery of BMP-2 and BMP-7 for bone regeneration using a heparinized collagen membrane

Abstract To investigate the effect of the sequential delivery of bone morphogenetic proteins BMP-2 and BMP-7 on bone regeneration in rat calvarial defects (40 Sprague-Dawley rats, 8 mm defect size), all animals were treated with a hydroxyapatite (HA)/tricalcium phosphate (TCP) bone graft covered wit...

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Bibliographic Details
Published in:International journal of oral and maxillofacial surgery 2015-07, Vol.44 (7), p.921-928
Main Authors: Jo, J.-Y, Jeong, S.-I, Shin, Y.-M, Kang, S.-S, Kim, S.-E, Jeong, C.-M, Huh, J.-B
Format: Article
Language:English
Subjects:
Animals
Bone Morphogenetic Protein 2 - administration & dosage
Bone Morphogenetic Protein 2 - pharmacology
Bone Morphogenetic Protein 7 - administration & dosage
Bone Morphogenetic Protein 7 - pharmacology
bone morphogenetic proteins
bone regeneration
Bone Regeneration - drug effects
Bone Substitutes - pharmacology
Collagen - pharmacology
collagen membrane
Dentistry
Drug Delivery Systems
Durapatite - pharmacology
guided bone regeneration
heparin
Osteogenesis - drug effects
Rats
Rats, Sprague-Dawley
sequential delivery
Skull - surgery
Surgery
Wound Healing - drug effects
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Summary:Abstract To investigate the effect of the sequential delivery of bone morphogenetic proteins BMP-2 and BMP-7 on bone regeneration in rat calvarial defects (40 Sprague-Dawley rats, 8 mm defect size), all animals were treated with a hydroxyapatite (HA)/tricalcium phosphate (TCP) bone graft covered with a collagen membrane. The experimental groups were as follows: (1) control group: unmodified collagen (no treatment); (2) BMP-2 group: 5 μg of BMP-2; (3) hep-BMP-7 group: 5 μg BMP-7 chemically bound to heparinized collagen; and (4) BMP-2/hep-BMP-7 group: 2.5 μg BMP-7 bound to heparinized collagen and subsequently treated with 2.5 μg BMP-2. Defect healing was examined at 2 and 8 weeks after surgery. The BMP-2 group showed the largest new bone area at week 2 (29.3 ± 7.3%; P = 0.009); new bone areas in the hep-BMP-7 and BMP-2/hep-BMP-7 groups were similar (11.8 ± 3.4% and 12.9 ± 5.71%, respectively; P = 0.917). After 8 weeks, the BMP-2/hep-BMP-7 group showed the largest new bone area (43.3 ± 6.2%), followed by the BMP-2 and hep-BMP-7 groups ( P = 0.013). Accordingly, in comparison with single deliveries of BMP-2 and BMP-7, sequential delivery of BMP-2 and BMP-7 using a heparinized collagen membrane significantly induced new bone formation with a smaller quantity of BMP-2 in rat calvarial defects.
ISSN:0901-5027
1399-0020
DOI:10.1016/j.ijom.2015.02.015