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The Schizosaccharomyces pombe cdc5 super(+) gene encodes an essential protein with homology to c-Myb
The Schizosaccharomyces pombe cdc5 super(+) gene was identified in the first screen for cell division cycle mutants in this yeast. The cdc5 super(+) gene was reported to be required for nuclear division but because of its modest elongation and leaky nature at the non-permissive temperature, it was n...
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Published in: | The EMBO journal 1994-01, Vol.13 (2), p.471-483 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | The Schizosaccharomyces pombe cdc5 super(+) gene was identified in the first screen for cell division cycle mutants in this yeast. The cdc5 super(+) gene was reported to be required for nuclear division but because of its modest elongation and leaky nature at the non-permissive temperature, it was not investigated further. We report the characterization of the single allele of this gene, cdc5-120, in more detail. The mutant arrests with a 2N DNA content and a single interphase nucleus. Further genetic analyses suggest that cdc5 super(+) gene function is essential in the G sub(2) phase of the cell cycle. We have cloned and sequenced the cdc5 super(+) gene. The deduced protein sequence predicts that Cdc5 is an 87 kDa protein and contains a region sharing significant homology with the DNA binding domain of the Myb family of transcription factors. Deletion mapping of the cdc5 super(+) gene has shown that the N-terminal 232 amino acids of the protein, which contain the Myb-related region, are sufficient to complement the cdc5 super(ts) strain. A cdc5 null mutant was generated by homologous recombination. Haploid cells lacking cdc5 super(+) are inviable, indicating that cdc5 super(+) is an essential gene. A fusion protein consisting of bacterial glutathione S-transferase joined in-frame to the N-terminal 127 amino acids of the Cdc5 protein is able to bind to DNA cellulose at low salt concentrations. This evidence suggests that cdc5 super(+) might encode a transcription factor whose activity is required for cell cycle progression and growth during G sub(2). |
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ISSN: | 0261-4189 |