Genetic Architecture of Natural Variation in Rice Chlorophyll Content Revealed by a Genome-Wide Association Study

Chlorophyll content is one of the most important physiological traits as it is closely related to leaf photo- synthesis and crop yield potential. So far, few genes have been reported to be involved in natural variation of chlorophyll content in rice （Oryza sativa） and the extent of variations explor...

Full description

Saved in:
Bibliographic Details
Published in:Molecular plant 2015-06, Vol.8 (6), p.946-957
Main Authors: Wang, Quanxiu, Xie, Weibo, Xing, Hongkun, Yan, Ju, Meng, Xiangzhou, Li, Xinglei, Fu, Xiangkui, Xu, Jiuyue, Lian, Xingming, Yu, Sibin, Xing, Yongzhong, Wang, Gongwei
Format: Article
Language:English
Subjects:
chlorophyll
Chlorophyll - metabolism
Gene Expression Regulation, Plant
Genetic Variation
Genome, Plant
Genome-Wide Association Study
Ghd7
GWAS
NAL1
Oryza
Oryza - classification
Oryza - genetics
Oryza - metabolism
Oryza sativa
Plant Proteins - genetics
pleiotropic effect
全基因组
单核苷酸多态性
叶绿素含量
水稻育种
生物合成基因
自然变异
遗传结构
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chlorophyll content is one of the most important physiological traits as it is closely related to leaf photo- synthesis and crop yield potential. So far, few genes have been reported to be involved in natural variation of chlorophyll content in rice （Oryza sativa） and the extent of variations explored is very limited. We con- ducted a genome-wide association study （GWAS） using a diverse worldwide collection of 529 O. sativa accessions. A total of 46 significant association loci were identified. Three F2 mapping populations with parents selected from the association panel were tested for validation of GWAS signals. We clearly demon- strated that Grain number, plant height, andheading date7 （GhdT） was a major locus for natural variation of chlorophyll content at the heading stage by combining evidence from near-isogenic lines and transgenic plants. The enhanced expression of Ghd7 decreased the chlorophyll content, mainly through down- regulating the expression of genes involved in the biosynthesis of chlorophyll and chloroplast. In addition, Narrow leaf1 （NAL1） corresponded to one significant association region repeatedly detected over two years. We revealed a high degree of polymorphism in the 5＇ UTR and four non-synonymous SNPs in the cod- ing region of NAL1, and observed diverse effects of the major haplotypes. The loci or candidate genes iden- tified would help to fine-tune and optimize the antenna size of canopies in rice breeding.
ISSN:1674-2052
1752-9867
DOI:10.1016/j.molp.2015.02.014