Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection

Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection

•Determination of the total content of some SAs in milk using SPE and a color reaction.•Solid-phase extraction of sulfonamides on hypercrosslinked polystyrene.•Ease qualitative or naked-eye semiquantitative estimation of sulfonamides levels.•Direct preconcentration from whole milk and determination...

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Bibliographic Details
Published in:Food chemistry 2015-12, Vol.188, p.51-56
Main Authors: Dmitrienko, Stanislava G., Kochuk, Elena V., Tolmacheva, Veronika V., Apyari, Vladimir V., Zolotov, Yury A.
Format: Article
Language:eng
Subjects:
Animals
Cattle
Cinnamates - chemistry
Hypercrosslinked polystyrene
Milk
Milk - chemistry
p-Dimethylaminocinnamaldehyde
Solid Phase Extraction - methods
Solid-phase extraction
Spectrophotometry
Spectrophotometry - methods
Sulfonamides
Sulfonamides - adverse effects
Sulfonamides - analysis
Sulfonamides - chemistry
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Summary:•Determination of the total content of some SAs in milk using SPE and a color reaction.•Solid-phase extraction of sulfonamides on hypercrosslinked polystyrene.•Ease qualitative or naked-eye semiquantitative estimation of sulfonamides levels.•Direct preconcentration from whole milk and determination in the eluate. A simple screening method for isolation and determination of the total content of some sulfonamides in milk using solid-phase extraction and a color reaction is described. This procedure is based on SPE of sulfonamides on hypercrosslinked polystyrene, elution with acetonitrile and off-line derivatization with p-dimethylaminocinnamaldehyde in acetonitrile followed by spectrophotometric determination. The reaction produces intense violet–red color and can be easily used both for quantitation of sulfonamides using spectrophotometry and for naked-eye semi-quantitative estimation. Maximum absorption of the reaction product was determined at 540nm. The Lambert–Beer’s law was obeyed in the range of 0.07–3.0μgmL−1 in eluate, with the squared correlation coefficient (R2) of 0.9875–0.9995, and the relative standard deviation (RSD) of 3–4%. The limits of SAs detection using preconcentration were of 0.02–0.03μgmL−1. The proposed method can be recommended as a routine screening method for quantitation of sulfonamides in milk.
ISSN:0308-8146
1873-7072