Identification of the proteins in green cotton fiber using a proteomics-based approach

The proteome of 21 DPA (days post-anthesis) of green cotton fiber was analyzed using two-dimensional gel electrophoresis to yield the first reference proteome map. Of 220 individual spots that were excised and analyzed by MALDI-TOF/TOF MS, 156 were identified and cataloged according to their functio...

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Bibliographic Details
Published in:Biotechnology letters 2013-09, Vol.35 (9), p.1519-1523
Main Authors: Li, Yan-Jun, Wang, Fu-Xin, Wang, Ya-Qin, Liu, Yong-Chang, Zhang, Xin-Yu, Sun, Yu-Qiang, Sun, Jie
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
biosynthesis
Biotechnology
Carbohydrate metabolism
Cotton
Cotton Fiber
Cotton fibers
cytoskeletal proteins
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
energy metabolism
Gossypium - chemistry
Life Sciences
lint cotton
Microbiology
Original Research Paper
Pigments
Plant biology
Plant Proteins - analysis
Proteins
proteome
Proteome - analysis
Proteomics
Proteomics - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spots
Two dimensional
two-dimensional gel electrophoresis
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Summary:The proteome of 21 DPA (days post-anthesis) of green cotton fiber was analyzed using two-dimensional gel electrophoresis to yield the first reference proteome map. Of 220 individual spots that were excised and analyzed by MALDI-TOF/TOF MS, 156 were identified and cataloged according to their functions. Twelve classes of proteins were identified. Many of these proteins were related to carbohydrate metabolism and energy production. Other notable functional classes included oxidoreductase, cell wall-related and cytoskeleton proteins. This study gives a snapshot of the proteome in 21 DPA green cotton fiber and advances our understanding of molecular mechanisms related to pigment biosynthesis in green cotton fiber.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1227-1