Comparative effects of inorganic and organic nitrogen on the growth and microcystin production of Microcystis aeruginosa

Nitrogen causes the frequent occurrence of harmful algal blooms and possible microcystin production. The effects of ammonia and alanine (Ala) on the growth and microcystin production of Microcystis aeruginosa were investigated using an isotope tracer (¹⁵N). The results indicated that Ala was directl...

Full description

Saved in:
Bibliographic Details
Published in:World journal of microbiology & biotechnology 2015-05, Vol.31 (5), p.763-772
Main Authors: Yan, YangWei, Dai, RuiHua, Liu, Yan, Gao, JiaYi, Wu, XuanHao
Format: Article
Language:English
Subjects:
Alanine
Alanine - metabolism
Algae
algal blooms
Amino acids
Ammonia
Ammonia - metabolism
Analysis
Applied Microbiology
arginine
Biochemistry
Biomass
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Blooms
Chromatography
Cyanobacteria
Environmental Engineering/Biotechnology
Isotope Labeling
isotopes
Leucine
Life Sciences
Mass spectrometry
Microbiology
microcystin-LR
Microcystins - metabolism
Microcystis
Microcystis - growth & development
Microcystis - metabolism
Microcystis aeruginosa
Nitrogen
Nitrogen Isotopes - metabolism
Original Paper
Residues
Scientific imaging
Solvents
Studies
tandem mass spectrometry
Toxins
Water pollution
Water quality
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitrogen causes the frequent occurrence of harmful algal blooms and possible microcystin production. The effects of ammonia and alanine (Ala) on the growth and microcystin production of Microcystis aeruginosa were investigated using an isotope tracer (¹⁵N). The results indicated that Ala was directly used by M. aeruginosa and contributed to biomass formation amounting to 2.1 × 10⁷ cells mL⁻¹on day 48, compared with only 6.2 × 10⁶cells mL⁻¹from ammonia alone. Microcystin-LR production with Ala was less than that of ammonia, which peaked at 50.2 fg cell⁻¹on day 6. Liquid chromatographic analysis with tandem mass spectrometry of¹⁵N–microcystin-LR suggested that¹⁵N from ammonia was probably synthesized into the arginine residue. By contrast,¹⁵N from Ala was assimilated into the Ala, leucine, the iso-linked (2R,3S)-3-methylaspartic acid, arginine, and certain unusual C₂₀amino acid residues. The results represent the forward steps in the determination of the nitrogen forms that fuel toxin production and blooms.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-015-1829-y