OsARG encodes an arginase that plays critical roles in panicle development and grain production in rice

Nitrogen is a crucial nutrient for plant growth and development. Arginine is considered to be an important amino acid for nitrogen transport and storage, playing a crucial role during plant seedling development. However, little is known about the role of arginine in nitrogen remobilization at the re...

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Published in:The Plant journal : for cell and molecular biology 2013, Vol.73 (2), p.190-200
Main Authors: Ma, Xuefeng, Cheng, Zhijun, Qin, Ruizhen, Qiu, Yang, Heng, Yueqin, Yang, Hui, Ren, Yulong, Wang, Xiaole, Bi, Jingcui, Ma, Xiaoding, Zhang, Xin, Wang, Jiulin, Lei, Cailin, Guo, Xiuping, wang, Jie, Wu, Fuqing, Jiang, Ling, Wang, Haiyang, Wan, Jianmin
Format: Article
Language:English
Subjects:
arginase
Arginase - genetics
Arginase - metabolism
arginine
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Cloning, Molecular
crop production
Crop science
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - physiology
Genetic engineering
hydrolysis
inflorescences
metabolism
mitochondria
Molecular and cellular biology
molecular cloning
Molecular Sequence Data
mutant
mutants
Mutation
Nitrogen
Nitrogen - metabolism
Oryza - enzymology
Oryza - genetics
Oryza - growth & development
panicle development
phenotype
plant growth
Plant physiology and development
Plant Proteins - metabolism
Plant Stems - growth & development
Plants, Genetically Modified
Rice
rice (Oryza sativa)
Seasons
seed set
seedlings
Seeds - growth & development
transgenes
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Summary:Nitrogen is a crucial nutrient for plant growth and development. Arginine is considered to be an important amino acid for nitrogen transport and storage, playing a crucial role during plant seedling development. However, little is known about the role of arginine in nitrogen remobilization at the reproductive stage. We isolated a rice mutant nglf‐1 with reduced plant height, small panicle and grain size, and low seed‐setting rate (10% in nglf‐1 compared to 93% in wild‐type). Map‐based cloning revealed that the mutant phenotype was caused by loss of function of a gene (OsARG) encoding an arginine hydrolysis enzyme, which is consistent with arginine accumulation in the mutant. The phenotype was partially corrected supplying exogenous nitrogen, and fully corrected by expression of a wild‐type OsARG transgene. Over‐expression of OsARG in rice (cv. Kitaake) increased grain number per plant under nitrogen‐limited conditions. OsARG was ubiquitously expressed in various organs, but most strongly in developing panicles. The OsARG protein was localized in the mitochondria, consistent with other arginases. Our results suggest that the arginase encoded by OsARG, a key enzyme in Arg catabolism, plays a critical role during panicle development, especially under conditions of insufficient exogenous nitrogen. OsARG is a potential target for crop improvement.
ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313x.2012.05122.x