Tunable Hydrogel Thin Films from Reactive Synthetic Polymers as Potential Two-Dimensional Cell Scaffolds

This article describes the formation of cross-linked 10–200-nm-thick polymer hydrogel films by alternating the spin-coating of two mutually reactive polymers from organic solutions, followed by hydrolysis of the resulting multilayer film in aqueous buffer. Poly­(methyl vinyl ether-alt-maleic anhydri...

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Bibliographic Details
Published in:Langmuir 2015-05, Vol.31 (20), p.5623-5632
Main Authors: Goujon, Laurent J, Hariharan, Santosh, Sayyar, Bahareh, Burke, Nicholas A. D, Cranston, Emily D, Andrews, David W, Stöver, Harald D. H
Format: Article
Language:English
Subjects:
Animals
Cell Line
Epithelial Cells - cytology
Epithelial Cells - metabolism
Humans
Hydrogels - chemical synthesis
Hydrogels - chemistry
Mammary Glands, Human - cytology
Mammary Glands, Human - metabolism
Materials Testing - methods
Membranes, Artificial
Mice
Myoblasts - cytology
Myoblasts - metabolism
Tissue Scaffolds - chemistry
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Summary:This article describes the formation of cross-linked 10–200-nm-thick polymer hydrogel films by alternating the spin-coating of two mutually reactive polymers from organic solutions, followed by hydrolysis of the resulting multilayer film in aqueous buffer. Poly­(methyl vinyl ether-alt-maleic anhydride) (PMM) was deposited from acetonitrile solution, and poly­(N-3-aminopropylmethacrylamide-co-N-2-hydroxypropylmethacrylamide) (PAPM x , where x corresponds to the 3-aminopropylmethacrylamide content ranging from 10 to 100%) was deposited from methanol. Multilayer films were formed in up to 20 deposition cycles. The films cross-linked during formation by reaction between the amine groups of PAPM x and the anhydride groups of PMM. The resulting multilayer films were covalently postfunctionalized by exposure to fluoresceinamine, decylamine, d-glucamine, or fluorescently labeled PAPM x solutions prior to the hydrolysis of residual anhydride in aqueous PBS buffer. This allowed tuning the hydrophobicity of the film to give static water contact angles ranging from about 5 to 90°. Increasing the APM content in PAPM x from 10 to 100% led to apparent Young’s moduli from 300 to 700 kPa while retaining sufficient anhydride groups to allow postfunctionalization of the films. This allowed the resulting (PMM/PAPM x ) multilayer films to be turned into adhesion-promoting or antifouling surfaces for C2C12 mouse myoblasts and MCF 10A premalignant human mammary epithelial cells.
ISSN:0743-7463
1520-5827
DOI:10.1021/acs.langmuir.5b00376