Evaluating microbe-plant interactions and varietal differences for enhancing biocontrol efficacy in root rot disease challenged cotton crop

The performance of cyanobacteria and Trichoderma based biocontrol formulations was evaluated in two cotton varieties (Gossypium hirsutum F1861 and Gossypium arboreum CISA 310). Evaluation of mortality after 4 weeks revealed a significant reduction, particularly in G. hirsutum F1861, with values of 1...

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Bibliographic Details
Published in:European journal of plant pathology 2015-06, Vol.142 (2), p.345-362
Main Authors: Babu, Santosh, Bidyarani, Ngangom, Chopra, Preeti, Monga, Dilip, Kumar, Rishi, Prasanna, Radha, Kranthi, Sandhya, Saxena, Anil Kumar
Format: Article
Language:English
Subjects:
Agriculture
Anabaena laxa
biological control
biological control agents
Biomedical and Life Sciences
Calothrix
chitosanase
compost extracts
Cotton
Crops
Cyanobacteria
DNA
DNA fingerprinting
Ecology
endo-1,4-beta-glucanase
enzyme activity
Flowers & plants
genes
Gossypium arboreum
Gossypium hirsutum
Life Sciences
metagenomics
mortality
Plant diseases
Plant Pathology
Plant Sciences
rhizosphere
root rot
soil
Trichoderma
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Summary:The performance of cyanobacteria and Trichoderma based biocontrol formulations was evaluated in two cotton varieties (Gossypium hirsutum F1861 and Gossypium arboreum CISA 310). Evaluation of mortality after 4 weeks revealed a significant reduction, particularly in G. hirsutum F1861, with values of 13 % (lower by 2 % over the Trichoderma commercial biocontrol agent). The percent mortality after drenching with the compost tea prepared using respective formulations, ranged from 28 to 75 % in G. arboreum CISA 310, with significantly lower values of 6–37.3 % in G. hirsutum. The Anabaena laxa RPAN8 formulation showed the lowest mortality. The activity of hydrolytic enzymes—β-1, 3 glucanase (EGase EC 3.2.1.39), β-1, 4 glucanase (EGase EC, 3.2.1.4) and chitosanase (EC 3.2.1.99) showed a significant enhancement in the inoculated treatments (T1–T6), with Calothrix sp. being among the top ranked treatments in both varieties. Comparison of DNA fingerprints (HIP-TG profiles) of rhizospheric soil DNA with those of corresponding pure cultures revealed a high degree of similarity, confirming the colonization of inoculated organisms. An amplicon of 1000 bp was observed in the soil metagenomic PCR-DNA profiles from both varieties, which confirmed the presence of an endoglucanase gene. Comparative analyses of responses of the two varieties revealed that Gossypium hirsutum F1861 showed higher values of hydrolytic enzymes and available N in soil. On the other hand, microbial inoculation elicited higher levels of chitosanase and defense enzyme activity in Gossypium arboreum CISA 310. This represents a first report illustrating the significance of varietal responses in cotton in relation to the efficacy of microbial biocontrol formulations and their establishment in the rhizosphere.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-015-0619-6