New semi-synthesis of ubiquitin C-terminal conjugate with 7-amino-4-methylcoumarin

The ligation of peptide hydrazides at a Gly site carrying a removal auxiliary was found to be an efficient process. This technology was successfully used for the synthesis of ubiquitin C‐terminal conjugates. Recombinant Ub(1–75)‐NHNH2 was prepared through the hydrozinolysis of the Ub(1–75)‐intein fu...

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Bibliographic Details

Published in:	Journal of peptide science 2014-02, Vol.20 (2), p.102-107
Main Authors:	Li, Yi-Tong, Liang, Jun, Li, Jia-Bin, Fang, Ge-Min, Huang, Yong, Liu, Lei
Format:	Article
Language:	English
Subjects:	Chemistry Techniques, Synthetic - methods Coumarins - chemistry deubiquitinating enzymes Electrophoresis, Polyacrylamide Gel expressed protein ligation Fusion protein Molecular Structure native chemical ligation peptide hydrazides Peptides Recombinant Proteins - chemistry Recombinant Proteins - genetics ubiquitin Ubiquitin C - chemical synthesis Ubiquitin C - chemistry
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