Yarrowia lipolytica NCIM 3589, a tropical marine yeast, degrades bromoalkanes by an initial hydrolytic dehalogenation step

The widespread industrial use of organobromines which are known persistent organic pollutants has led to their accumulation in sediments and water bodies causing harm to animals and humans. While degradation of organochlorines by bacteria is well documented, information regarding degradation pathway...

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Bibliographic Details
Published in:Biodegradation (Dordrecht) 2015-04, Vol.26 (2), p.127-138
Main Authors: Vatsal, Aakanksha, Zinjarde, Smita S., Kumar, Ameeta Ravi
Format: Article
Language:English
Subjects:
Aerobiosis
Alcohols
Alcohols - metabolism
Aquatic Pollution
Bacterial Proteins - metabolism
Biodegradation
Biodegradation, Environmental
Biomedical and Life Sciences
Carbon dioxide
Carbon Dioxide - metabolism
Contaminated sediments
Environmental effects
Fatty acids
Fatty Acids - metabolism
Freshwater
Geochemistry
Hydrocarbons, Brominated - metabolism
Hydrolases - metabolism
Life Sciences
Microbiology
Organic contaminants
Organochlorine compounds
Original Paper
Pentanes - metabolism
Persistent organic pollutants
Pollutants
Seawater - chemistry
Soil Science & Conservation
Terrestrial Pollution
Waste Management/Waste Technology
Waste Water Technology
Water Management
Water Pollutants, Chemical - metabolism
Water Pollution Control
Yarrowia - metabolism
Yarrowia lipolytica
Yeast
Yeasts
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Summary:The widespread industrial use of organobromines which are known persistent organic pollutants has led to their accumulation in sediments and water bodies causing harm to animals and humans. While degradation of organochlorines by bacteria is well documented, information regarding degradation pathways of these recalcitrant organobromines is scarce. Hence, their fates and effects on the environment are of concern. The present study shows that a tropical marine yeast, Yarrowia lipolytica NCIM 3589 aerobically degrades bromoalkanes differing in carbon chain length and position of halogen substitution viz., 2-bromopropane (2-BP), 1-bromobutane (1-BB), 1,5 dibromopentane (1,5-DBP) and 1-bromodecane (1-BD) as seen by an increase in cell mass, release of bromide and concomitant decrease in concentration of brominated compound. The amount of bromoalkane degraded was 27.3, 21.9, 18.0 and 38.3 % with degradation rates of 0.076, 0.058, 0.046 and 0.117/day for 2-BP, 1-BB, 1,5-DBP and 1-BD, respectively. The initial product formed respectively were alcohols viz., 2-propanol, 1-butanol, 1-bromo, 5-pentanol and 1-decanol as detected by GC–MS. These were further metabolized to fatty acids viz., 2-propionic, 1-butyric and 1-decanoic acid eventually leading to carbon dioxide formation. Neither higher chain nor brominated fatty acids were detected. An inducible extracellular dehalogenase responsible for removal of bromide was detected with activities of 21.07, 18.82, 18.96 and 26.67 U/ml for 2-BP, 1-BB, 1,5-DBP and 1-BD, respectively. We report here for the first time the proposed aerobic pathway of bromoalkane degradation by an eukaryotic microbe Y. lipolytica 3589, involving an initial hydrolytic dehalogenation step.
ISSN:0923-9820
1572-9729
DOI:10.1007/s10532-015-9721-x