Toscana virus meningitis case in Switzerland: an example of the ezVIR bioinformatics pipeline utility for the identification of emerging viruses

Toscana virus (TOSV) represents a frequent cause of viral meningitis in the Mediterranean Basin that remains neglected in neighbouring countries. We report a documented TOSV meningitis case in a traveller returning from Tuscany to Switzerland. While routine serological and PCR assays could not discr...

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Bibliographic Details
Published in:Clinical microbiology and infection 2015-04, Vol.21 (4), p.387.e1-387.e4
Main Authors: Cordey, S., Bel, M., Petty, T.J., Docquier, M., Sacco, L., Turin, L., Cherpillod, P., Emonet, S., Louis-Simonet, M., Zdobnov, E.M., Ambrosioni, J., Kaiser, L.
Format: Article
Language:English
Subjects:
Adolescent
Bunyaviridae Infections - diagnosis
Bunyaviridae Infections - pathology
Cerebrospinal Fluid - virology
Computational Biology
Diagnostic, ezVIR
high-throughput sequencing
Humans
Male
meningitis
Meningitis - diagnosis
Meningitis - pathology
Middle Aged
Polymerase Chain Reaction
Sandfly fever Naples virus - classification
Sandfly fever Naples virus - genetics
Sandfly fever Naples virus - isolation & purification
Sequence Analysis, DNA
Switzerland
Toscana virus
Young Adult
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Summary:Toscana virus (TOSV) represents a frequent cause of viral meningitis in the Mediterranean Basin that remains neglected in neighbouring countries. We report a documented TOSV meningitis case in a traveller returning from Tuscany to Switzerland. While routine serological and PCR assays could not discriminate between TOSV and Sandfly fever Naples virus infection, a high-throughput sequencing performed directly on the cerebrospinal fluid specimen and analysed with the ezVIR pipeline provided an unequivocal viral diagnostic. TOSV could be unequivocally considered as the aetiological agent, proving the potential of ezVIR to improve standard diagnostics in cases of infection with uncommon or emerging viruses.
ISSN:1198-743X
1469-0691
DOI:10.1016/j.cmi.2014.11.010