The comet assay: a comprehensive review

The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using...

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Bibliographic Details
Published in:Mutation research. Reviews in genetic toxicology 1995-02, Vol.339 (1), p.37-59
Main Authors: Fairbairn, Daryl W., Olive, Peggy L., O'Neill, Kim L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical mutagenesis
Comet assay
DNA single strand breaks
Double strand breaks
Medical sciences
Microgel electrophoresis
Single cell gel
Toxicology
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Summary:The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented. Applications in different fields are reviewed and possible future directions of the technique are briefly explored.
ISSN:0165-1110
DOI:10.1016/0165-1110(94)00013-3