Long-term storage of aerobic granules in liquid media: Viable but non-culturable status

[Display omitted] •Aerobic granules were stored in liquid media at 4°C for over one year.•Liquid media included DI water, acetone, acetone/isoamyl acetate, formaldehyde.•The reactivated granules had high bioactivity and nice strength.•Microbial communities of reactivated granules were probed.•Cells...

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Bibliographic Details
Published in:Bioresource technology 2014-08, Vol.166, p.464-470
Main Authors: Wan, Chunli, Zhang, Qinlan, Lee, Duu-Jong, Wang, Yayi, Li, Jieni
Format: Article
Language:English
Subjects:
Acetone
Actinobacteria
Activation
Aerobic granules
Aerobiosis
Biological and medical sciences
Bioreactors - microbiology
Culture Media
Culture Techniques
Flavobacteria
Fundamental and applied biological sciences. Psychology
Granular materials
Granules
High throughput sequencing
Liquids
Media
Saline water
Sequencing
Signaling molecules
Storage
Viable but non-culturable status
Waste Water - chemistry
Water Purification - methods
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Summary:[Display omitted] •Aerobic granules were stored in liquid media at 4°C for over one year.•Liquid media included DI water, acetone, acetone/isoamyl acetate, formaldehyde.•The reactivated granules had high bioactivity and nice strength.•Microbial communities of reactivated granules were probed.•Cells secreted c-di-GMP and ppGpp to enter viable but non-culturable status. Long-term storage and successful reactivation after storage are essential for practical applications of aerobic granules on wastewater treatment. This study cultivated aerobic granules (SI) in sequencing batch reactors and then stored the granules at 4°C in five liquid media (DI water (SW), acetone (SA), acetone/isoamyl acetate mix (SAA), saline water (SS), and formaldehyde (SF)) for over 1year. The first four granules were then successfully reactivated in 24h cultivation. The specific oxygen uptake rates (SOUR) of the granules followed SI>SS>SA>SAA>SW>SF; and the corresponding granular strengths (10min ultrasound) followed SI>SA=SS>SAA>SW>>SF. During storage the granular cells secreted excess quantities of cyclic-diguanylate (c-di-GMP) and pentaphosphate (ppGpp) as responses to the stringent challenges. We proposed that to force cells in granules (Alphaproteobacteria, Flavobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Sphingobacteria, and Clostridia) entering viable but non-culturable (VBNC) status is the key of success for extended period storage of granules.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2014.05.091