Glycopolymer Functionalization of Engineered Spider Silk Protein-based Materials for Improved Cell Adhesion

Silk protein‐based materials are promising biomaterials for application as tissue scaffolds, due to their processability, biocompatibility, and biodegradability. The preparation of films composed of an engineered spider silk protein (eADF4(C16)) and their functionalization with glycopolymers are des...

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Bibliographic Details
Published in:Macromolecular bioscience 2014-07, Vol.14 (7), p.936-942
Main Authors: Hardy, John G., Pfaff, André, Leal-Egaña, Aldo, Müller, Axel H. E., Scheibel, Thomas R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biocompatibility
Biocompatible Materials - pharmacology
biodegradable
biomaterials
Biomedical materials
biomimetics
Biopolymers - chemistry
Biopolymers - pharmacology
Cell adhesion
Cell adhesion & migration
Cell Adhesion - drug effects
Coating
coatings
Fibroblasts - cytology
Fibroblasts - drug effects
Glycopolymers
Mice
Molecular Sequence Data
Polysaccharides - chemical synthesis
Polysaccharides - chemistry
Polysaccharides - pharmacology
Protein Binding - drug effects
Protein Engineering
Proteins
Rats
Scaffolds
Silk
Silk - chemistry
Silk - pharmacology
Spiders
Spiders - chemistry
Surface Properties
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Summary:Silk protein‐based materials are promising biomaterials for application as tissue scaffolds, due to their processability, biocompatibility, and biodegradability. The preparation of films composed of an engineered spider silk protein (eADF4(C16)) and their functionalization with glycopolymers are described. The glycopolymers bind proteins found in the extracellular matrix, providing a biomimetic coating on the films that improves cell adhesion to the surfaces of engineered spider silk films. Such silk‐based materials have potential as coatings for degradable implantable devices. Recombinantly produced spider silk protein‐based materials are promising for use as tissue scaffolds. Herein, the preparation of films functionalized with extracellular matrix protein‐binding glycopolymers, and their potential as biocompatible coatings, are reported.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201400020