Study of neuron survival on polypyrrole-embedded single-walled carbon nanotube substrates for long-term growth conditions

Cultures of primary embryonic rat brain hippocampus neurons with supporting glia cells were carried out on different substrates containing polypyrrole (PPy) and/or single‐walled carbon nanotubes (SWCNTs). Neuron adhesion, neurites and dendrites branching elongation, and development of neuron network...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2014-12, Vol.102 (12), p.4443-4454
Main Authors: Hernández-Ferrer, Javier, Pérez-Bruzón, Rodolfo N., Azanza, María J., González, Mónica, Del Moral, Raquel, Ansón-Casaos, Alejandro, de la Fuente, Jesús M., Marijuan, Pedro C., Martínez, M. Teresa
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Adhesion - drug effects
Cell Culture Techniques
Cell Survival - drug effects
Cells, Cultured
Cross-disciplinary physics: materials science
rheology
Culture
cytotoxicity
Dendritic structure
Deposition
Exact sciences and technology
Hippocampus - cytology
Hippocampus - metabolism
long-term growth conditions
Materials science
Materials Testing
Medical sciences
Nanoscale materials and structures: fabrication and characterization
Nanostructure
Nanotubes
Nanotubes, Carbon - chemistry
Networks
neural growth
Neuroglia - cytology
Neuroglia - metabolism
Neurons
Neurons - cytology
Neurons - metabolism
Physics
Polymers - chemistry
Polymers - pharmacology
polypyrrole electrodeposition
Pyrroles - chemistry
Pyrroles - pharmacology
Rats
Single wall carbon nanotubes
single-walled carbon nanotubes
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Survival
Technology. Biomaterials. Equipments
Time Factors
Viability
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Summary:Cultures of primary embryonic rat brain hippocampus neurons with supporting glia cells were carried out on different substrates containing polypyrrole (PPy) and/or single‐walled carbon nanotubes (SWCNTs). Neuron adhesion, neurites and dendrites branching elongation, and development of neuron networks on substrates were followed by phase‐contrast optical microscopy and quantified to state cell survival and proliferation. Suspensions of as‐grown and purified SWCNTs were sprayed on a glass coverslips and PPy/SWCNTs were deposited by potentiodynamic electrochemical deposition. Cell neurotoxicity revealed by neuron death was very high for purified SWCNTs substrates in good agreement with [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] (MTT) test showing lower viability on SWCNTs containing substrates compared with PPy‐substrates and control samples probably due to the metal content and the carboxylic groups introduced during the purification. It is interesting to highlight that neurons grown on PPy‐substrates adhere developing neurites and branching dendrites earlier even than on control cultures. On subsequent days the neurons are able to adapt to nanotube substrates developing neuron networks for 14‐day cultures with similar patterns of complexity for control, PPy and PPy/SWCNT substrates. PPy/SWCNT substrates show a lower impedance value at frequencies under 1 Hz. We have come to the conclusion that glia cells and PPy added to the culture medium and substrates respectively, improve in some degree nanotube biocompatibility, cell adhesion and hence cell viability. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4443–4454, 2014.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.35110