Use of random amplified polymorphic DNA (RAPD) markers in the discrimination and verification of genotypes in Eucalyptus

Four separate studies were carried out using random amplified polymorphic DNA (RAPD) markers to analyse samples of Eucalyptus supplied by several different organisations. The objective was to examine the reproducibility of the RAPD technique and its ability to discriminate between individual genotyp...

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Bibliographic Details
Published in:Theoretical and applied genetics 1994-10, Vol.89 (4), p.442-450
Main Authors: KEIL, M, GRIFFIN, A. R
Format: Article
Language:English
Subjects:
adn
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
dna
eucalyptus
Eucalyptus camaldulensis
Eucalyptus grandis
Eucalyptus nitens
Fundamental and applied biological sciences. Psychology
genetic markers
genetic polymorphism
Genetics of eukaryotes. Biological and molecular evolution
genotipos
genotype
genotypes
marcadores geneticos
marqueur genetique
nucleotide sequence
polimorfismo genetico
polymorphisme genetique
Pteridophyta, spermatophyta
secuencia nucleica
sequence nucleique
Vegetals
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Summary:Four separate studies were carried out using random amplified polymorphic DNA (RAPD) markers to analyse samples of Eucalyptus supplied by several different organisations. The objective was to examine the reproducibility of the RAPD technique and its ability to discriminate between individual genotypes for verification of clonal identities. It was found that RAPD profiles that are unique to a genotype can be generated reliably and simply and that even closely related genotypes can be distinguished. In addition, in each of the four studies, cases were detected where the plant material studied had been mis-sampled or mislabelled (i.e. the RAPD profiles were not consistent with the identification numbers): ramets of a Eucalyptus grandis clone were found to be derived from 2 different clones; ramets labelled as 2 different Eucalyptus hybrid clones were found to be the same clone, owing to a mis-planted clonal hedge; samples supplied as a single progeny of a controlled Eucalyptus nitens cross were derived from two crosses involving different pairs of parents; mislabelling was detected for ramets of 4 of a set of 10 clones of E. grandis and Eucalyptus camaldulensis. For three of the four studies, the detection of genotype mis-identifications was unexpected, suggesting that labelling or sampling errors during the handling of plant material are a frequent occurrence, with potentially serious economic consequences.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00225379