StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

We have shown previously that the potato dehydration responsive element binding (StDREB1) transcription factor plays an important role in regulating and improving salt and drought stress-response genes in potato plant. To further characterize StDREB1 involvement in stress response, we focuse here on...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2015-04, Vol.121 (1), p.237-248
Main Authors: Bouaziz, Donia, Jbir, Rania, Charfeddine, Safa, Saidi, Mohamed Najib, Gargouri-Bouzid, Radhia
Format: Article
Language:English
Subjects:
Abiotic stress
Antioxidants
Biomedical and Life Sciences
Catalase
Cellular stress response
copper
Dehydration
Drought
Electrophoretic mobility
Enzymatic activity
enzyme activity
gel electrophoresis
Gene expression
Genes
Hydrogen peroxide
Life Sciences
Malondialdehyde
organ culture
Original Paper
osmotolerance
Oxidative stress
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Potatoes
Regulatory sequences
Salinity tolerance
salt stress
salt tolerance
Salts
signal transduction
Solanum tuberosum
Stress response
Superoxide dismutase
Transcription activation
Transcription factors
Transgenic plants
Zinc
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Summary:We have shown previously that the potato dehydration responsive element binding (StDREB1) transcription factor plays an important role in regulating and improving salt and drought stress-response genes in potato plant. To further characterize StDREB1 involvement in stress response, we focuse here on the investigation of the StDREB1 target genes by an Electrophoretic mobility shift assay. The data obtains indicated that the StDREB1 protein can bind to both GCC and DRE boxes in the promoter sequence of target genes, suggesting that this transcription factor may play a key role in the response to abiotic- and biotic-stresses by the activation of the DRE- and GCC- mediated signaling pathways. In a second step, since some DREB factors were related to the oxidative stress response, we showed that H₂O₂treatment led to a significant increase of StDREB1 expression in wild- type potato plants. Moreover, the analysis of the oxidative stress response of StDREB1 transgenic potato plants revealed lower levels of H₂O₂and malondialdehyde than wild-type control plants submitted in vitro to salt stress. An increase in the antioxidant enzyme activities including superoxide dismutase (SOD) and catalase was also observed in StDREB1 plants. In addition, an enhanced expression of the Cu/Zn SOD gene was noticed in these StDREB1 transgenic plants, cultivated under salt stress conditions. These results suggest that StDREB1 plays an essential role in the regulation of stress-response by regulating the oxidative stress response. The involvement of this transcription factor in the activation of osmoprotectant synthesis was also confirmed.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-014-0698-7