Cloning and characterization of the abortive infection genetic determinant abiD isolated from pBF61 of Lactococcus lactis subsp. lactis KR5

A 6.3-kb fragment from pBF61 in Lactococcus lactis subsp. lactis KR5 was cloned and found to confer an abortive phage infection (Abi+) phenotype exhibiting a reduction in efficiency of plating and plaque size for small isometric- and prolate-headed bacteriophages sk1 and c2, respectively, and to pro...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1995-05, Vol.61 (5), p.2023-2026
Main Authors: McLandsborough, L.A. (University of Minnesota, Minneapolis, MN.), Kolaetis, K.M, Requena, T, McKay, L.L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
BACTERIOFAGOS
Bacteriology
BACTERIOPHAGE
Bacteriophages - growth & development
Base Sequence
Biological and medical sciences
Biology
CLONACION
CLONAGE
Cloning, Molecular
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Bacterial
Genetics
LACTOCOCCUS LACTIS
Lactococcus lactis - genetics
Lactococcus lactis - virology
Microbiology
Molecular Sequence Data
Mutagenesis, Insertional
Open Reading Frames
PATHOGENESE
PATOGENESIS
Phenotype
PLASMIDE
PLASMIDIOS
Plasmids - genetics
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
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Description
Summary:A 6.3-kb fragment from pBF61 in Lactococcus lactis subsp. lactis KR5 was cloned and found to confer an abortive phage infection (Abi+) phenotype exhibiting a reduction in efficiency of plating and plaque size for small isometric- and prolate-headed bacteriophages sk1 and c2, respectively, and to produce a 10-fold decrease in c2 phage burst size. Phage adsorption was not significantly reduced. An open reading frame of 1,098 bp was sequenced and designated abiD. Tn5 mutagenesis confirmed that abiD was required for the Abi+ phenotype
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.61.5.2023-2026.1995