Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. PCR a...

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Bibliographic Details
Published in:Future microbiology 2015-04, Vol.10 (4), p.527-535
Main Authors: Rahman, Md Masudur, Noor, Monira, Islam, Kazi Mehetazul, Uddin, Md Bashir, Hossain, Ferdaus Mohd Altaf, Zinnah, Mohammad Ali, Mamun, Mohammad Al, Islam, Mohammad Rafiqul, Eo, Seong Kug, Ashour, Hossam M
Format: Article
Language:English
Subjects:
Animals
Bacteriological Techniques - methods
Bovine tuberculosis
Cattle
Diagnosis
Health aspects
Humans
Methods
Milk
Milk - microbiology
molecular diagnosis
Molecular diagnostic techniques
Molecular Diagnostic Techniques - methods
Mycobacterium bovis - genetics
Mycobacterium bovis - isolation & purification
Polymerase Chain Reaction - methods
Risk Factors
Sputum - microbiology
Tuberculosis
Tuberculosis, Bovine - diagnosis
Tuberculosis, Bovine - microbiology
Tuberculosis, Pulmonary - diagnosis
Tuberculosis, Pulmonary - microbiology
Zoonoses
zoonotic transmission
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Summary:To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. PCR amplification of the targeted DNA fragment of was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb.14.139