High numbers of macrophages, especially M2-like (CD163-positive), correlate with hyaluronan accumulation and poor outcome in breast cancer

Aims High amounts of tumour‐associated macrophages (TAMs) and hyaluronan (HA) correlate with tumour aggressiveness in breast cancer, but the relationship between these parameters is unclear. The aim of this study was to assay the numbers of TAMs in 278 human breast cancer cases, and their correlatio...

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Bibliographic Details
Published in:Histopathology 2015-05, Vol.66 (6), p.873-883
Main Authors: Tiainen, Satu, Tumelius, Ritva, Rilla, Kirsi, Hämäläinen, Kirsi, Tammi, Markku, Tammi, Raija, Kosma, Veli-Matti, Oikari, Sanna, Auvinen, Päivi
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, CD - analysis
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - analysis
Antigens, Differentiation, Myelomonocytic - metabolism
Breast cancer
Breast Neoplasms - mortality
Breast Neoplasms - pathology
CD163
CD68
Disease Progression
Female
Humans
Hyaluronan
hyaluronan synthase
Hyaluronic Acid - metabolism
Immunohistochemistry
In Situ Hybridization
Kaplan-Meier Estimate
Macrophages - pathology
Medical research
Middle Aged
obesity
Obesity - complications
Prognosis
Receptors, Cell Surface - analysis
tumour-associated macrophage
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Summary:Aims High amounts of tumour‐associated macrophages (TAMs) and hyaluronan (HA) correlate with tumour aggressiveness in breast cancer, but the relationship between these parameters is unclear. The aim of this study was to assay the numbers of TAMs in 278 human breast cancer cases, and their correlations with HA‐related factors, clinical variables, and outcome. Methods and results The immunoreactivities for CD163 and CD68 were considered as indicators for M2‐like and all TAMs, respectively. The numbers of TAMs were counted in at least four hot spots, and averaged to represent the numbers of TAMs in each section. In the statistical analyses, the numbers were graded as either low or high according to the median. High numbers of TAMs correlated with a high tumour HA content, HA synthases, CD44 positivity, and poor outcome. The number of CD163‐positive cells represented a strong independent prognostic factor. There was also a significant correlation between obesity and a high number of CD163‐positive cells. Conclusions Concurrent increases in TAMs and HA in breast cancer indicate that the accumulation of HA facilitates macrophage infiltration and inflammatory responses during human breast cancer progression.
ISSN:0309-0167
1365-2559
DOI:10.1111/his.12607