In vivo effects of 17 beta -estradiol on cardiac Na super(+)/K super(+)-ATPase expression and activity in rat heart

In this study the in vivo effects of estradiol in regulating Na super(+)/K super(+)-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, ca...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular endocrinology 2014-05, Vol.388 (1-2), p.58-68
Main Authors: Obradovic, Milan, Stewart, Alan J, Pitt, Samantha J, Labudovic-Borovic, Milica, Sudar, Emina, Petrovic, Voin, Zafirovic, Sonja, Maravic-Stojkovic, Vera, Vasic, Vesna, Isenovic, Esma R
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Heart
In vivo testing
In vivo tests
Kinases
Rats
Surgical implants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study the in vivo effects of estradiol in regulating Na super(+)/K super(+)-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na super(+)/K super(+)-ATPase activity, expression of the alpha 1 subunit, and phosphorylation of the alpha 1 subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na super(+) levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na super(+)/K super(+)-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na super(+)/K super(+)-ATPase expression and activity in rat heart.
ISSN:0303-7207
DOI:10.1016/j.mce.2014.03.005