Micro-solid phase extraction of ochratoxin A, and its determination in urine using capillary electrophoresis

We describe a simple, environmentally friendly and selective technique for the determination of ochratoxin A (OTA) in urine. It involves (a) the use of a molecularly imprinted polymer as a sorbent in micro-solid-phase extraction in which the sorbent is contained in a propylene membrane envelope, and...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2013-08, Vol.180 (11-12), p.1149-1156
Main Authors: Lee, Tien Ping, Saad, Bahruddin, Salleh, Baharuddin, Mat, Ishak
Format: Article
Language:English
Subjects:
Analytical Chemistry
Capillarity
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electrophoresis
Extraction
Imprinted polymers
Microengineering
Nanochemistry
Nanotechnology
Original Paper
Propylene
Sorbents
Standard deviation
Urine
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Summary:We describe a simple, environmentally friendly and selective technique for the determination of ochratoxin A (OTA) in urine. It involves (a) the use of a molecularly imprinted polymer as a sorbent in micro-solid-phase extraction in which the sorbent is contained in a propylene membrane envelope, and (b) separation and detection by capillary electrophoresis (CE). Under optimized conditions, response is linear in the range between 50 and 300 ng mL −1 (with a correlation coefficient of 0.9989), relative standard deviations range from 4 to 8 %, the detection limit for OTA in urine is 11.2 ng mL −1 (with a quantification limits of 32.5 ng mL −1 ) which is lower than those of previously reported methods for solid-phase extraction combined with CE. The recoveries of OTA from urine spiked at levels of 50, 150 and 300 ng mL −1 ranged from 93 to 97 %. Figure ᅟ
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-013-1042-3