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Hyphal vacuolation and fragmentation in Penicillium chrysogenum
A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation an...
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| Published in: | Biotechnology and bioengineering 1994-01, Vol.44 (5), p.655-660 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 660 |
| container_issue | 5 |
| container_start_page | 655 |
| container_title | Biotechnology and bioengineering |
| container_volume | 44 |
| creator | Paul, G.C Kent, C.A Thomas, C.R |
| description | A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation and the fall of the mean total and main hyphal lengths suggests that hyphal fragmentation is not necessarily due to "shear" alone. The physiological state of the hyphae, characterized by the proportions of vacuoles, was found to have a significant influence on the breakage of mycelial hyphae. It was found that the fragmentation was greater when the hyphae became heavily vacuolated following nutrient limitation in the culture, i.e., during the switch from the rapid growth to the production phase. |
| doi_str_mv | 10.1002/bit.260440513 |
| format | article |
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Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation and the fall of the mean total and main hyphal lengths suggests that hyphal fragmentation is not necessarily due to "shear" alone. The physiological state of the hyphae, characterized by the proportions of vacuoles, was found to have a significant influence on the breakage of mycelial hyphae. It was found that the fragmentation was greater when the hyphae became heavily vacuolated following nutrient limitation in the culture, i.e., during the switch from the rapid growth to the production phase.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260440513</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>New York, NY: Wiley</publisher><subject>Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; cell culture ; cell suspension culture ; fermentation ; fragmentation ; Fundamental and applied biological sciences. Psychology ; hyphae ; Microbiology ; Mission oriented research ; Morphology, structure, chemical composition ; Mycology ; Penicillium chrysogenum ; Physiology and metabolism ; vacuoles</subject><ispartof>Biotechnology and bioengineering, 1994-01, Vol.44 (5), p.655-660</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4206029$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Paul, G.C</creatorcontrib><creatorcontrib>Kent, C.A</creatorcontrib><creatorcontrib>Thomas, C.R</creatorcontrib><title>Hyphal vacuolation and fragmentation in Penicillium chrysogenum</title><title>Biotechnology and bioengineering</title><description>A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation and the fall of the mean total and main hyphal lengths suggests that hyphal fragmentation is not necessarily due to "shear" alone. The physiological state of the hyphae, characterized by the proportions of vacuoles, was found to have a significant influence on the breakage of mycelial hyphae. It was found that the fragmentation was greater when the hyphae became heavily vacuolated following nutrient limitation in the culture, i.e., during the switch from the rapid growth to the production phase.</description><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>cell culture</subject><subject>cell suspension culture</subject><subject>fermentation</subject><subject>fragmentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hyphae</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Morphology, structure, chemical composition</subject><subject>Mycology</subject><subject>Penicillium chrysogenum</subject><subject>Physiology and metabolism</subject><subject>vacuoles</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNotkM1Lw0AQxRdRsFaPns1BvEVnPzJJTiJFrVBQ0J7DdLvbrmw2NZsI_e9daU_DzPsxvPcYu-ZwzwHEw8oN9wJBKSi4PGETDnWZg6jhlE0AAHNZ1OKcXcT4ndayQpywx_l-tyWf_ZIeO0-D60JGYZ3ZnjatCcPh4kL2YYLTzns3tpne9vvYbUwY20t2ZslHc3WcU7Z8ef6azfPF--vb7GmRWwHFkMs1lIrQ1GDXWolqBQTWaPvv1aLBWpeysFoXpVaq4sAJURrSAiskSuKU3R3-7vruZzRxaFoXtfGegunG2HAsuSgEJPD2CFLU5FOOoF1sdr1rqd83SgCmShJ2c8AsdQ1t-oQsPwVwCVzVSqYC_wAyIGLQ</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Paul, G.C</creator><creator>Kent, C.A</creator><creator>Thomas, C.R</creator><general>Wiley</general><scope>FBQ</scope><scope>IQODW</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19940101</creationdate><title>Hyphal vacuolation and fragmentation in Penicillium chrysogenum</title><author>Paul, G.C ; Kent, C.A ; Thomas, C.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f205t-3d074a6e90fdc428b0a0fecf4405f6e69c735fcc57c448101a663eac2686aac73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>cell culture</topic><topic>cell suspension culture</topic><topic>fermentation</topic><topic>fragmentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hyphae</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Morphology, structure, chemical composition</topic><topic>Mycology</topic><topic>Penicillium chrysogenum</topic><topic>Physiology and metabolism</topic><topic>vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paul, G.C</creatorcontrib><creatorcontrib>Kent, C.A</creatorcontrib><creatorcontrib>Thomas, C.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paul, G.C</au><au>Kent, C.A</au><au>Thomas, C.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyphal vacuolation and fragmentation in Penicillium chrysogenum</atitle><jtitle>Biotechnology and bioengineering</jtitle><date>1994-01-01</date><risdate>1994</risdate><volume>44</volume><issue>5</issue><spage>655</spage><epage>660</epage><pages>655-660</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation and the fall of the mean total and main hyphal lengths suggests that hyphal fragmentation is not necessarily due to "shear" alone. The physiological state of the hyphae, characterized by the proportions of vacuoles, was found to have a significant influence on the breakage of mycelial hyphae. It was found that the fragmentation was greater when the hyphae became heavily vacuolated following nutrient limitation in the culture, i.e., during the switch from the rapid growth to the production phase.</abstract><cop>New York, NY</cop><pub>Wiley</pub><doi>10.1002/bit.260440513</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3592 |
| ispartof | Biotechnology and bioengineering, 1994-01, Vol.44 (5), p.655-660 |
| issn | 0006-3592 1097-0290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16712520 |
| source | Wiley-Blackwell Journals (Backfile Content) |
| subjects | Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology cell culture cell suspension culture fermentation fragmentation Fundamental and applied biological sciences. Psychology hyphae Microbiology Mission oriented research Morphology, structure, chemical composition Mycology Penicillium chrysogenum Physiology and metabolism vacuoles |
| title | Hyphal vacuolation and fragmentation in Penicillium chrysogenum |
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