Hyphal vacuolation and fragmentation in Penicillium chrysogenum

A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation an...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1994-01, Vol.44 (5), p.655-660
Main Authors: Paul, G.C, Kent, C.A, Thomas, C.R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
cell culture
cell suspension culture
fermentation
fragmentation
Fundamental and applied biological sciences. Psychology
hyphae
Microbiology
Mission oriented research
Morphology, structure, chemical composition
Mycology
Penicillium chrysogenum
Physiology and metabolism
vacuoles
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Summary:A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vacuolation and morphology was obtained by image analysis. In fed-batch fermentations the coincidence of the events of rapid vacuolation and the fall of the mean total and main hyphal lengths suggests that hyphal fragmentation is not necessarily due to "shear" alone. The physiological state of the hyphae, characterized by the proportions of vacuoles, was found to have a significant influence on the breakage of mycelial hyphae. It was found that the fragmentation was greater when the hyphae became heavily vacuolated following nutrient limitation in the culture, i.e., during the switch from the rapid growth to the production phase.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260440513