Accurate approach to capillary-supported optical diffraction tomography

A new holographic data processing path for accurate quantitative tomographic reconstruction of 3D samples placed in a cylindrical capillary is proposed. The method considers strong unintentional focusing effects induced by the inner cylindrical boundary of the vessel: 1) introduction of cylindrical...

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Bibliographic Details
Published in:Optics express 2015-03, Vol.23 (6), p.7908-7923
Main Authors: Kostencka, Julianna, Kozacki, Tomasz, Kuś, Arkadiusz, Kujawińska, Małgorzata
Format: Article
Language:English
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Summary:A new holographic data processing path for accurate quantitative tomographic reconstruction of 3D samples placed in a cylindrical capillary is proposed. The method considers strong unintentional focusing effects induced by the inner cylindrical boundary of the vessel: 1) introduction of cylindrical wave illumination of a sample, and 2) object wave deformation. The first issue is addressed by developing an arbitrary illumination tomographic reconstruction algorithm based on filtered backpropagation, while the second by a novel correction algorithm utilizing the optical rays analysis. Moreover, the processing path includes a novel holographic method for correction of spherical aberration related to refraction at a planar surface. Utility of the developed data processing path is proven with numerical simulations and experimental measurement of a specially prepared test sample.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.23.007908