Changes in lymphocytes induced by oxidation substrates

Lymphocytes in blood smears of children diagnosed with cancer have been investigated and compared to those of healthy children (matching controls). The conventional hematological procedure for smear staining was used to assess the blood formula and lymphocyte indices, and a new staining method invol...

Full description

Saved in:
Bibliographic Details
Published in:Biophysics (Oxford) 2014, Vol.59 (6), p.894-899
Main Authors: Khunderyakova, N. V., Plyasunova, S. A., Litvinova, E. G., Yatchkula, T. V., Zakharchenko, M. V., Kovsan, A. V., Fedotcheva, N. I., Schwartsburd, P. M., Kondrashova, M. N.
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biophysics
Cancer
Cell Biophysics
Children & youth
Dehydrogenases
Enzymes
Oncology
Oxidation
Physics
Physics and Astronomy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lymphocytes in blood smears of children diagnosed with cancer have been investigated and compared to those of healthy children (matching controls). The conventional hematological procedure for smear staining was used to assess the blood formula and lymphocyte indices, and a new staining method involving incubation of cells in a biochemical medium for 1 h was used to assess the activity of succinate dehydrogenase, lactate dehydrogenase, and the ratio of the activities of these enzymes characterizing the interaction of respiration and glycolysis. A dramatic increase of the size of lymphocytes obtained from cancer patients was detected when lactic acid was added to the incubation medium for the biochemical reaction. This effect was neither observed when succinic acid was added nor when blood smears from healthy children of the control group were tested. Structural changes in the patients’ cells were associated with changes in the activity of the enzymes under investigation. The cytobiochemical test that was developed allows increasing diagnostic sensitivity upon assessment of the patients’ state and can contribute to more efficient treatment of malignant tumors.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350914060050