Evaluation of PCR in the diagnosis of canine leishmaniasis in two different epidemiological regions: Campinas (SP) and Teresina (PI), Brazil

Using the polymerase chain reaction (PCR) test for diagnosis of canine leishmaniasis has greater sensitivity and specificity than culture and visualization of the parasite. This study compares PCR for the diagnosis of the genus and species of Leishmania with serological techniques used for the contr...

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Bibliographic Details
Published in:Epidemiology and infection 2015-04, Vol.143 (5), p.1088-1095
Main Authors: COSTA, L. N. G., BORBA, A. S., CASTAGNA, C. L., CARVALHO FILHO, E. B., MARSON, F. A. L., SÁ JUNIOR, F. F., ANGERAMI, R. N., LEVY, C. E.
Format: Article
Language:English
Subjects:
Animals
Brazil
DNA, Protozoan - analysis
Dog Diseases - diagnosis
Dogs
Enzyme-Linked Immunosorbent Assay - veterinary
Gene amplification
Immunoassay
Infections
Leishmania - genetics
Leishmania - isolation & purification
Leishmania chagasi
Leishmania infantum - genetics
Leishmania infantum - isolation & purification
Leishmaniasis - diagnosis
Leishmaniasis - veterinary
Leishmaniasis, Visceral - diagnosis
Leishmaniasis, Visceral - veterinary
Original Papers
Other infections
Parasites
Parasitic diseases
Polymerase Chain Reaction - veterinary
Protozoa
Sensitivity and Specificity
Sequence Analysis, DNA - veterinary
Serology
Zoonoses
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Summary:Using the polymerase chain reaction (PCR) test for diagnosis of canine leishmaniasis has greater sensitivity and specificity than culture and visualization of the parasite. This study compares PCR for the diagnosis of the genus and species of Leishmania with serological techniques used for the control of canine visceral leishmaniasis (CVL) in Brazil, considering two regions. We analysed peripheral blood samples collected from 195 dogs in the Campinas (SP) and Teresina (PI) regions. ELISA was performed as a serological method and PCR was performed using specific primers for the genus Leishmania spp. and the species Leishmania chagasi. In Campinas, a greater sensitivity of PCR (88·24%) (P = 0·0455) compared to Teresina (14·71%) (P 
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268814001721