Abnormal spermatogenesis and reduced fertility in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus

•IE180 of pseudorabies virus is multifunctional transcription factor.•IE180 transgenic mice showed male infertility with abnormal spermatogenesis.•IE180 induced morphological, numerical, and functional anomalies of spermatozoa.•IE180 was detected in the male germinal cells of pseudorabies virus infe...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2013-11, Vol.440 (4), p.683-688
Main Authors: Tomioka, Yukiko, Morimatsu, Masami, Taharaguchi, Satoshi, Yamamoto, Sayo, Suyama, Haruka, Ozaki, Kinuyo, Iwamori, Naoki, Ono, Etsuro
Format: Article
Language:English
Subjects:
Alphaherpesvirinae
Animals
Fertility
Herpesvirus 1, Suid
Immediate-early protein
Immediate-Early Proteins - genetics
Infertility, Male - pathology
Infertility, Male - virology
Male
Mice, Transgenic
Organ Size
Pseudorabies - complications
Pseudorabies - pathology
Pseudorabies virus
Spermatogenesis
Spermatogenesis - genetics
Testis - pathology
Testis - virology
Transgenic mice
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Summary:•IE180 of pseudorabies virus is multifunctional transcription factor.•IE180 transgenic mice showed male infertility with abnormal spermatogenesis.•IE180 induced morphological, numerical, and functional anomalies of spermatozoa.•IE180 was detected in the male germinal cells of pseudorabies virus infected mice. Transcription factors of alphaherpesviruses not only control the expression of their own viral genes, but also influence the gene expression of mammalian cells. In the course of breeding of the transgenic mouse line (TgIE96) expressing the immediate-early protein IE180 of pseudorabies virus belonging to the subfamily Alphaherpesvirinae, we found that TgIE96 male mice suffered from severe breeding difficulties. Testes of TgIE96 were smaller than that of non-transgenic littermates and abnormal spermatogenesis such as morphological, numerical and functional anomalies of spermatozoa were found in the transgenic mouse line. Expression of IE180 was detected in the germ cells at all stages, especially spermatocytes, and fewer Sertoli cells. In addition, expression of IE180 was also detected in the germinal cells of C57BL/6 mice inoculated with PRV into their testes. These results suggest that IE180 of PRV induces male infertility by abnormal spermatogenesis, which effect morphological, numerical, and functional anomalies of spermatozoa, in transgenic mice.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2013.09.125