EBV-miR-BART1 is involved in regulating metabolism-associated genes in nasopharyngeal carcinoma

•EBV-miR-BART1 can modulate metabolism associated genes in NPC cells.•EBV-miR-BART1 is associated with NPC metabolism.•EBV-miR-BART1 may play important roles in the development of NPC. EBV-miR-BART1 has been found to be highly expressed in some cancers including nasopharyngeal carcinoma (NPC), but i...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2013-06, Vol.436 (1), p.19-24
Main Authors: Ye, Yanfen, Zhou, Ying, Zhang, Lan, Chen, Yuxiang, Lyu, Xiaoming, Cai, Longmei, Lu, Yaoyong, Deng, Yi, Wang, Jianguo, Yao, Kaitai, Fang, Weiyi, Cai, Hongbing, Li, Xin
Format: Article
Language:English
Subjects:
Biopsy
Carcinoma
Carrier Proteins - metabolism
Cell Line, Tumor
DNA, Viral - analysis
EBV-miR-BART1
Epstein-Barr Virus Infections - metabolism
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Herpesvirus 4, Human - metabolism
High-Throughput Nucleotide Sequencing
Humans
Metabolism-associated genes
MicroRNAs - metabolism
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms - metabolism
Nasopharyngeal Neoplasms - virology
Nasopharyngitis - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•EBV-miR-BART1 can modulate metabolism associated genes in NPC cells.•EBV-miR-BART1 is associated with NPC metabolism.•EBV-miR-BART1 may play important roles in the development of NPC. EBV-miR-BART1 has been found to be highly expressed in some cancers including nasopharyngeal carcinoma (NPC), but its exact roles in the pathogenesis of NPC remain unclear. Here, we did RNA deep sequencing to compare the gene expression profile between EBV-miR-BART1-expressing CNE1 cells and the control cells to determine the possible effects of EBV-miR-BART1 in NPC. Gene expression profiling analysis unexpectedly showed a significant number of up- and down-modulated metabolism-associated genes, such as G6PD, SAT1, ASS1, PAST1, FUT1, SGPL1, DHRS3, B4GALT1, PHGDH, IDH2, PISD, UGT8, LDHB and GALNT1, in EBV-miR-BART1-expressing NPC cells, which were next confirmed by RT-qPCR. Moreover, of these metabolism-genes, PSAT1 and PHGDH expression levels were significantly upregulated and most of other genes were obviously up-expressed in NPC specimens compared with chronic nasopharyngitis (CNP) tissues. Collectively, we for the first time found the effects of EBV-miR-BART1 on the expression of mechanism-associated genes in NPC, suggesting a novel role of EBV-miR-BART1 in cancer metabolism, which remains to be fully elucidated.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2013.05.008