Application of 16S rRNA metagenomics to analyze bacterial communities at a respiratory care centre in Taiwan

In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectiv...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 2015-03, Vol.99 (6), p.2871-2881
Main Authors: Tang, Chuan Yi, Yiu, Siu-Ming, Kuo, Han-Yueh, Tan, Te-Sheng, Liao, Ki-Hok, Liu, Chih-Chin, Hon, Wing-Kai, Liou, Ming-Li
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter baumannii
Acinetobacter baumannii - classification
Acinetobacter baumannii - drug effects
Alleles
Analysis
Bacteria
Bacteria - classification
Bacteria - drug effects
bacterial communities
Biomass
Biomedical and Life Sciences
Biomedical research
Biotechnology
carbapenems
Carbapenems - pharmacology
Chryseobacterium - classification
Chryseobacterium - drug effects
compliance
Computer science
DNA, Bacterial - genetics
Drug resistance
Drug Resistance, Multiple, Bacterial
Enterococcus - classification
Enterococcus - drug effects
Environmental Biotechnology
Equipment Contamination
Fomites - microbiology
Genetic aspects
Genomics
guidelines
Hospitals
Humans
hygiene
Intensive care
Klebsiella pneumoniae - classification
Klebsiella pneumoniae - drug effects
Laboratories
Life Sciences
Medical equipment
metagenomics
Metagenomics - methods
Microbial Genetics and Genomics
Microbial Sensitivity Tests
Microbiology
multidimensional scaling
Nosocomial infections
Pathogens
phylotype
Pseudomonas aeruginosa - classification
Pseudomonas aeruginosa - drug effects
Respiratory failure
Respiratory therapy
Respiratory tract diseases
Ribosomal RNA
risk
RNA, Ribosomal, 16S - genetics
sanitation
Sequence Analysis, DNA
Staphylococcus
Staphylococcus - classification
Staphylococcus - drug effects
Staphylococcus infections
Studies
Taiwan
Ventilators
Work stations
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during the sampling period in the RCC. A high amount of microbial diversity was detected, with an average of 1,836 phylotypes per sample. In addition to Acinetobacter, more than 60 % of the bacterial communities present among the top 25 abundant genera were dominated by skin-associated bacteria. Differences in bacterial profiles were restricted to individual samples. Furthermore, compliance with hand hygiene guidelines may be unsatisfactory among hospital staff according to a principal coordinate analysis that indicated clustering of bacterial communities between devices and workstations for most of the sampling sites. Compared to the high incidence of clinical isolates in the RCC, only Staphylococcus and Acinetobacter were highly abundant in the IHEs. Despite Acinetobacter was the most abundant genus present in IHEs of the RCC, potential pathogens, e.g., Acinetobacter baumannii, might remain susceptible to carbapenem. This study is the first in Taiwan to demonstrate a high diversity of human-associated bacteria in the RCC via 16S rRNA metagenomics, which allows for new assessment of potential health risks in RCCs, aids in the evaluation of existing sanitation protocols, and furthers our understanding of the development of healthcare-associated infections.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-014-6176-7