Changes in cell size and number and in rhizodermal development contribute to root tip swelling of Hyoscyamus albus roots subjected to iron deficiency

Root tip swelling is a common phenomenon observed when plant roots are subjected to Fe deficiency. We analysed whether an increase in cell number or an enlargement of cell width was involved in this phenomenon. Root tips of Hyoscyamus albus cultured with or without Fe were stained with fluorescent S...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology and biochemistry 2015-04, Vol.89, p.107-111
Main Authors: Kawahara, Yuki, Kitamura, Yoshie
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Cell number
Cell Proliferation
Cell Size
Confocal laser-scanning microscopy
Fe deficiency
Hyoscyamus - growth & development
Hyoscyamus - metabolism
Hyoscyamus albus
Iron - deficiency
Iron - metabolism
Meristem - growth & development
Microscopy, Confocal - methods
Plant Cells
Plant Epidermis - growth & development
Plant Roots - growth & development
Plant Roots - metabolism
Root hair
Root morphology
Stress, Physiological
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Root tip swelling is a common phenomenon observed when plant roots are subjected to Fe deficiency. We analysed whether an increase in cell number or an enlargement of cell width was involved in this phenomenon. Root tips of Hyoscyamus albus cultured with or without Fe were stained with fluorescent SYTO14 and analysed by confocal laser-scanning microscopy. Time-course and position-based examination revealed that the inhibition of longitudinal cell elongation and acceleration of transverse cell enlargement under Fe deficiency started from the tips and then extended towards the base during the time-course period. An increase in cell number also occurred behind the tips. In addition, the development of rhizodermal protrusions was observed on the surface of roots subjected to Fe deficiency. These results indicated that changes in cell size and number and in root hair development were all involved in root tip swelling. [Display omitted] •We analyse cell size and number in swelling root tip under Fe deficiency.•Inhibition of cell elongation and acceleration of cell enlargement are observed.•An increase in cell number and root hair development also occur.•The changes begin from the tip and extend to the base.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2015.02.018