Microbial Synthesis of Plant Oxylipins from γ‑Linolenic Acid through Designed Biotransformation Pathways

Secondary metabolites of plants are often difficult to synthesize in high yields because of the large complexity of the biosynthetic pathways and challenges encountered in the functional expression of the required biosynthetic enzymes in microbial cells. In this study, the biosynthesis of plant oxyl...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2015-03, Vol.63 (10), p.2773-2781
Main Authors: Kim, Sae-Um, Kim, Kyoung-Rok, Kim, Ji-Won, Kim, Soomin, Kwon, Yong-Uk, Oh, Deok-Kun, Park, Jin-Byung
Format: Article
Language:English
Subjects:
Biosynthetic Pathways
Biotransformation
Escherichia coli - genetics
Escherichia coli - metabolism
gamma-Linolenic Acid - metabolism
Metabolic Engineering
Oxylipins - metabolism
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Summary:Secondary metabolites of plants are often difficult to synthesize in high yields because of the large complexity of the biosynthetic pathways and challenges encountered in the functional expression of the required biosynthetic enzymes in microbial cells. In this study, the biosynthesis of plant oxylipinsa family of oxygenated unsaturated carboxylic acidswas explored to enable a high-yield production through a designed microbial synthetic system harboring a set of microbial enzymes (i.e., fatty acid double-bond hydratases, alcohol dehydrogenases, Baeyer–Villiger monooxygenases, and esterases) to produce a variety of unsaturated carboxylic acids from γ-linolenic acid. The whole cell system of the recombinant Escherichia coli efficiently produced (6Z,9Z)-12-hydroxydodeca-6,9-dienoic acid (7), (Z)-9-hydroxynon-6-enoic acid (15), (Z)-dec-4-enedioic acid (17), and (6Z,9Z)-13-hydroxyoctadeca-6,9-dienoic acid (2). This study demonstrated that various secondary metabolites of plants can be produced by implementing artificial biosynthetic pathways into whole-cell biocatalysis.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf5058843