Murine Oligodendroglial Cells Express Nerve Growth Factor

The studies reported here present evidence for the expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) by an oligodendroglial cell line and of NGF by oligodendrocytes in mouse primary culture. An immortalized oligodendroglial cell line (N19) expressing markers for im...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1994-09, Vol.91 (19), p.8812-8816
Main Authors: Byravan, Sujatha, Foster, Lyndon M., Phan, Tommy, Verity, A. Neil, Campagnoni, Anthony T.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Antibodies
Base Sequence
Brain
Brain-Derived Neurotrophic Factor
Cell Differentiation
Cell lines
Cellular biology
DNA Primers - chemistry
Gene Expression
In Situ Hybridization
Messenger RNA
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nerve Growth Factors - metabolism
Nerve Tissue Proteins - metabolism
Nervous system
Neural stem cells
Neurites - ultrastructure
Neuroglia
Neurons
Oligodendroglia
Oligodendroglia - metabolism
PC12 Cells
RNA, Messenger - genetics
Rodents
Schwann cells
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Summary:The studies reported here present evidence for the expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) by an oligodendroglial cell line and of NGF by oligodendrocytes in mouse primary culture. An immortalized oligodendroglial cell line (N19) expressing markers for immature oligodendrocytes stimulated PC12 cells to elaborate processes. Polymerase chain reaction analysis with degenerate primers indicated that the N19 cells expressed the mRNAs for the neurotrophic factors NGF and BDNF. Northern blot analysis confirmed that the N19 cells expressed the 1.3-kb NGF mRNA and the 1.4- and 4-kb BDNF mRNAs. In situ hybridization histochemistry identified the presence of NGF mRNAs in 9-day primary oligodendroglial cultures. Combined immunocytochemistry and in situ hybridization histochemistry colocalized NGF mRNA within primary cultured cells that immunostained for the oligodendrocyte marker galactocerebroside (GC). Double-immunofluorescence analysis also colocalized NGF protein within GC+cells and within A2B5 +cells, a marker for oligodendrocyte progenitors. These results show that oligodendroglia and their precursor cells can express the neurotrophic factor NGF. They suggest that cells in the oligodendrocyte lineage may play an active role in neurite extension through fiber tracts in addition to myelination.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.19.8812