Effects of curine in HL-60 leukemic cells: cell cycle arrest and apoptosis induction

Curine is a natural alkaloid isolated from Chondrodendron platyphyllum and it has been reported that this alkaloid has vasodilatory and anti-inflammatory effects. The aim of this study is to analyze the cytotoxic effects of curine in cancer cell lines HL-60, K562, and HT-29, and in primary cultures...

Full description

Saved in:
Bibliographic Details
Published in:Journal of natural medicines 2015-04, Vol.69 (2), p.218-223
Main Authors: Dantas, Bruna Braga, Faheina-Martins, Gláucia Veríssimo, Coulidiati, Tangbadioa Hervé, Bomfim, Caio César Barbosa, da Silva Dias, Celidarque, Barbosa-Filho, José Maria, Araújo, Demetrius Antônio Machado
Format: Article
Language:English
Subjects:
Annexin A5
Antineoplastic Agents, Phytogenic - isolation & purification
Antineoplastic Agents, Phytogenic - pharmacology
Antineoplastic Agents, Phytogenic - therapeutic use
Apoptosis - drug effects
Biomedical and Life Sciences
Biomedicine
Cell Cycle Checkpoints - drug effects
Cell Survival - drug effects
Complementary & Alternative Medicine
HL-60 Cells
HT29 Cells
Humans
Isoquinolines - isolation & purification
Isoquinolines - pharmacology
Isoquinolines - therapeutic use
Leukemia, Promyelocytic, Acute - drug therapy
Leukocytes, Mononuclear - drug effects
Medicinal Chemistry
Membrane Potential, Mitochondrial - drug effects
Menispermaceae - chemistry
Original Paper
Pharmacology/Toxicology
Pharmacy
Phytotherapy
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Plant Sciences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Curine is a natural alkaloid isolated from Chondrodendron platyphyllum and it has been reported that this alkaloid has vasodilatory and anti-inflammatory effects. The aim of this study is to analyze the cytotoxic effects of curine in cancer cell lines HL-60, K562, and HT-29, and in primary cultures of peripheral blood mononuclear cells (PBMC). Cells were treated with curine (from 3 to 15 µM) for 24 and 48 h. Cell viability was analyzed by the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and flow cytometry with propidium iodide (PI) assay. To assess the type of cell death induced in HL-60, the cell cycle, morphological, and biochemical alterations were analyzed, which were determined by differential staining with acridine orange/ethidium bromide, and annexin V/PI double-labeling and change in mitochondrial membrane potential assays. Curine demonstrated a potent cytotoxic effect on leukemic cell lines (HL-60 and K562). Its cytotoxic effects in HL-60 cells was related to plasma membrane damage and cell cycle arrest at the G1 phase from 43.4 ± 1.0 to 56.7 ± 1.4 % ( p  
ISSN:1340-3443
1861-0293
DOI:10.1007/s11418-014-0881-5