Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction

A sensitive assay based on amplification of a 319-bp DNA fragment of the intracellular bacterium of swine proliferative enteritis was developed for the detection of the organism in the feces of swine. A vernacular name, ileal symbiont intracellularis (IS-intracellularis), has recently been published...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1993-10, Vol.31 (10), p.2611-2615
Main Authors: Jones, G.F, Ward, G.E, Murtaugh, M.P, Lin, G, Gebhart, C.J
Format: Article
Language:English
Subjects:
Animals
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Campylobacter - genetics
Campylobacter - isolation & purification
CERDO
DIAGNOSTIC
DIAGNOSTICO
DNA, Bacterial - analysis
ENTERITE
ENTERITIS
Enteritis - microbiology
Enteritis - veterinary
FECES
Feces - microbiology
Fundamental and applied biological sciences. Psychology
HECES
Microbiology
Molecular Sequence Data
Polymerase Chain Reaction
PORCIN
Swine
Swine Diseases - microbiology
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Summary:A sensitive assay based on amplification of a 319-bp DNA fragment of the intracellular bacterium of swine proliferative enteritis was developed for the detection of the organism in the feces of swine. A vernacular name, ileal symbiont intracellularis (IS-intracellularis), has recently been published for the intracellular bacterium, which was formerly known as a Campylobacter-like organism (C.J. Gebhart, S. M. Barnes, S. McOrist, G.F. Lin, and G. H. K. Larson, Int. J. Syst. Bacteriol. 43:533-538, 1993). As few as 10(2) IS-intracellularis organisms purified from intestinal mucosa, or 10(3) IS-intracellularis per g of feces, were detected. No amplification product was produced from a polymerase chain reaction performed on DNA extracted from the feces of healthy pigs. A 319-bp DNA fragment specific for IS-intracellularis was produced on amplification of DNA from the feces of pigs with experimental and naturally occurring proliferative enteritis
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.31.10.2611-2615.1993