Immunogenicity and protective effect of recombinant Brucella abortus Ndk (rNdk) against a virulent strain B. abortus 544 infection in BALB/c mice

In this study, we particularly evaluated the protective effect of recombinant protein encoded by Brucella abortus 544 ndk (nucleoside diphosphate kinase) gene against B. abortus infection in the BALB/c mice. Cloning and expression of B. abortus Ndk was accomplished by PCR amplification into a pMAL e...

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Bibliographic Details
Published in:FEMS microbiology letters 2015-02, Vol.362 (4), p.1-6
Main Authors: Hop, Huynh Tan, Simborio, Hannah Leah, Reyes, Alisha Wehdnesday Bernardo, Arayan, Lauren Togonon, Min, WonGi, Lee, Hu Jang, Kim, Dong Hee, Chang, Hong Hee, Kim, Suk
Format: Article
Language:English
Subjects:
Animals
Brucella abortus
Brucella abortus - genetics
Brucella abortus - immunology
Brucellosis
Brucellosis - immunology
Brucellosis - prevention & control
Cloning
Cloning, Molecular
Cytokines
Effectiveness studies
Elk
Enzyme-Linked Immunosorbent Assay
Immune response (cell-mediated)
Immunity, Cellular
Immunity, Humoral
Immunization
Immunogenicity
Immunoglobulin G
Immunoglobulin G - biosynthesis
Immunoglobulin G - immunology
Infections
Inflammation
Interferon-gamma - immunology
Interferon-gamma - metabolism
Interleukin 10
Interleukin 6
Interleukin-10 - immunology
Interleukin-10 - metabolism
Interleukin-6 - immunology
Interleukin-6 - metabolism
Kinases
Mice
Mice, Inbred BALB C
Microbiology
Nucleoside-diphosphate kinase
Nucleoside-Diphosphate Kinase - genetics
Nucleoside-Diphosphate Kinase - immunology
Recombinant Proteins - isolation & purification
Spleen
Tumor necrosis factor
Tumor Necrosis Factor-alpha - immunology
Tumor Necrosis Factor-alpha - metabolism
Vaccines
Vaccines, Subunit
γ-Interferon
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Summary:In this study, we particularly evaluated the protective effect of recombinant protein encoded by Brucella abortus 544 ndk (nucleoside diphosphate kinase) gene against B. abortus infection in the BALB/c mice. Cloning and expression of B. abortus Ndk was accomplished by PCR amplification into a pMAL expression system, and purification of a recombinant Ndk (rNdk). As for the determination of IgG responses, rNdk induced vigorous IgG production, especially higher in IgG2a compared to IgG1 with titers of 5.2 and 4.8, respectively, whereas titers of these in mice immunized with MBP were 2.4 of IgG2a and 2.6 of IgG1. The analysis of cytokine has revealed that rNdk can strongly induce production of IFN-γ as well as proinflammatory cytokines (TNF, MCP1 and IL-6) but not much IL-10, suggesting rNdk elicited predominantly cell-mediated immune responses. Furthermore, the spleen proliferation and bacterial burden in the spleen of rNdk immunized mice were significantly lower than those of MBP-immunized mice against virulent B. abortus challenge (P < 0.01). Conclusionly, rNdk immunization enables to elicit both of the humoral and cellular response, ultimately enhancing protection level in experimental mice, suggesting that rNdk of B. abortus might be a useful candidate for subunit vaccine for brucellosis in animals. The immunization with rNdk enables to elicit markedly both of the humoral and cellular response, and enhance protection level against virulent Brucella abortus challenge in mice.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnv003