Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for int...

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Bibliographic Details
Published in:Brain tumor pathology 2015-01, Vol.32 (1), p.12-19
Main Authors: Tanino, Mishie, Sasajima, Toshio, Nanjo, Hiroshi, Akesaka, Shiori, Kagaya, Masami, Kimura, Taichi, Ishida, Yusuke, Oda, Masaya, Takahashi, Masataka, Sugawara, Taku, Yoshioka, Toshiaki, Nishihara, Hiroshi, Akagami, Yoichi, Goto, Akiteru, Minamiya, Yoshihiro, Tanaka, Shinya
Format: Article
Language:English
Subjects:
Accuracy
Adolescent
Adult
Aged
Aged, 80 and over
Antibodies
Antigen-Antibody Reactions
Biomarkers, Tumor - analysis
Brain cancer
Brain Neoplasms - diagnosis
Brain Neoplasms - pathology
Cancer Research
Cloning
Diagnosis, Differential
Electric fields
Electricity
Female
Frozen Sections
Glioma - diagnosis
Glioma - pathology
Humans
Immunohistochemistry - methods
Intraoperative Period
Ki-67 Antigen - analysis
Lymphoma
Lymphoma - diagnosis
Lymphoma - pathology
Male
Medicine
Medicine & Public Health
Metastasis
Middle Aged
Neoplasm Staging
Neurology
Neurosurgery
Oncology
Original Article
Pathology
Sensitivity and Specificity
Stains & staining
Tumors
Young Adult
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Description
Summary:Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
ISSN:1433-7398
1861-387X
DOI:10.1007/s10014-014-0188-y