Protective Effect of Mildronate against Toxic Influence of Mercury(II) Chloride on Cultured Neuroblastoma Cells

We examined toxic action of 10.9 μM mercury(II) chloride on cultured IMR-32 neuroblastoma cells and possible protective effects of a cardioprotector, mildronate (tested concentrations 0.01 to 10.0 mg/ml of culture medium) under such experimental conditions. Isolated addition of mildronate to the med...

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Bibliographic Details
Published in:Neurophysiology (New York) 2014-06, Vol.46 (3), p.271-273
Main Authors: Sokurenko, L. M., Chaikovskii, Yu. B.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cell culture
Mercury
Neurobiology
Neuroblastoma
Neurosciences
Toxicity
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Summary:We examined toxic action of 10.9 μM mercury(II) chloride on cultured IMR-32 neuroblastoma cells and possible protective effects of a cardioprotector, mildronate (tested concentrations 0.01 to 10.0 mg/ml of culture medium) under such experimental conditions. Isolated addition of mildronate to the medium did not induce considerable negative effects (number of dead cells did not exceed 8-10% of their total number at all concentrations used). In the case of isolated action of HgCl 2 , about half of cultured IMR- 32 cells died (on average, 55.8 ± 1.6% of the cells remained viable). Mildronate in a 0.01 mg/ml concentration did not manifest a significant protective action, but at doses 1.0 and 10.0 mg/ml the mean number of living cells in the presence of HgCl 2 reached nearly 80%. Thus, mildronate demonstrated relative safety in vitro and a significant protective effect under conditions of intoxication by low doses of mercury.
ISSN:0090-2977
1573-9007
DOI:10.1007/s11062-014-9440-7