Conditioned medium of adipose-derived stromal cell culture in three-dimensional bioreactors for enhanced wound healing

Abstract Background It was previously shown that human adipose-derived stromal cell (hADSC)-conditioned medium (CM) promotes wound healing. An essential part of the wound healing process is neovascularization in the wound bed. Materials and methods We hypothesized that CM prepared from hADSCs cultur...

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Bibliographic Details
Published in:The Journal of surgical research 2015-03, Vol.194 (1), p.8-17
Main Authors: Kwon, Sun Hyun, MS, Bhang, Suk Ho, PhD, Jang, Hyeon-Ki, BS, Rhim, Taiyoun, PhD, Kim, Byung-Soo, PhD
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
Bioreactors
Cells, Cultured
Conditioned medium
Culture Media, Conditioned
Female
Human adipose-derived stromal cells
Humans
Mice
Mice, Inbred BALB C
Neovascularization
Neovascularization, Physiologic
Spheroids, Cellular
Stromal Cells - physiology
Surgery
Wound Healing
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Summary:Abstract Background It was previously shown that human adipose-derived stromal cell (hADSC)-conditioned medium (CM) promotes wound healing. An essential part of the wound healing process is neovascularization in the wound bed. Materials and methods We hypothesized that CM prepared from hADSCs cultured as spheroids in three-dimensional suspension bioreactors (spheroid CM) would contain much higher concentrations of angiogenic growth factors secreted by hADSCs, induce a higher extent of neovascularization in the wound bed, and improve wound healing as compared with CM prepared by conventional monolayer culture (monolayer CM). Results The concentrations of angiogenic growth factors (i.e., vascular endothelial growth factor, basic fibroblast growth factor, and hepatocyte growth factor) in spheroid CM were 20- to 145-fold higher than those in monolayer CM. Either fresh medium, monolayer CM, or spheroid CM was administered to full-thickness wounds created on the dorsal aspects of athymic mice. The monolayer CM promoted wound healing as compared with fresh medium or no treatment. Importantly, wound closure was faster, and dermal and epidermal regeneration was improved in the spheroid CM-treated mice compared with that in the monolayer CM-treated mice. Conclusions The improved wound healing by spheroid CM may be attributed, at least in part, to enhanced neovascularization in the wound beds. The spheroid-based CM approach showed potential as a therapy for skin wound repair.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2014.10.053