Emission of ESBL/AmpC-producing Escherichia coli from pig fattening farms to surrounding areas

•First systematic study on transmission of ESBL/AmpC – Escherichia coli between pig farms and their surroundings.•High detection of ESBL/AmpC – E. coli in slurry; low detection in barn air and ambient air.•ESBL/AmpC – E. coli found on surfaces in the vicinity.•Emission via slurry and transmission vi...

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Bibliographic Details
Published in:Veterinary microbiology 2015-01, Vol.175 (1), p.77-84
Main Authors: von Salviati, Christina, Laube, Henriette, Guerra, Beatriz, Roesler, Uwe, Friese, Anika
Format: Article
Language:English
Subjects:
Animal Husbandry
Animals
Antimicrobial resistance
Bacterial Proteins - genetics
beta-Lactam Resistance
beta-Lactamases - genetics
Cluster Analysis
Diptera
Dogs
Electrophoresis, Gel, Pulsed-Field - veterinary
Emission
Environment
ESBL
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Feces - microbiology
Manure - microbiology
Mice
Microbial Sensitivity Tests - veterinary
Pig husbandry
Slurry
Swine
Swine Diseases - microbiology
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Summary:•First systematic study on transmission of ESBL/AmpC – Escherichia coli between pig farms and their surroundings.•High detection of ESBL/AmpC – E. coli in slurry; low detection in barn air and ambient air.•ESBL/AmpC – E. coli found on surfaces in the vicinity.•Emission via slurry and transmission via flies proven by PFGE. The presence of ESBL/AmpC-producing Escherichia coli in livestock such as pigs has been known for some time. However, to date there is little information about the transmission of these resistant bacteria between pig farms and their surroundings. Thus, the aim of this study was to explore this topic by investigating seven German pig fattening farms. Samples from outside (including ground surfaces, ambient air, slurry and digestate from biogas plants) and, in parallel, from inside the pig barns (including pig feces, dust, barn air, flies and mice feces) were examined for ESBL/AmpC-producing E. coli and selected isolates were compared by pulsed-field gel electrophoresis (PFGE) analysis. 14/17 (82.4%) slurry samples and three of four samples of digestate from biogas plants tested positive for ESBL/AmpC-producing E. coli. In the vicinity of the pig barns these resistant bacteria were detected in 14/87 (16.1%) boot swabs taken from various ground surfaces and in 2/36 (6%) ambient air samples. Inside the pig barns, 6/63 (9.5%) barn air samples and a small proportion of flies and mice feces samples were ESBL/AmpC-positive. PFGE analysis proved fecal emission as well as a possible spread via flies, as identical ESBL-E. coli isolates were detected in slurry and on fertilized fields, as well as in flies and pooled feces from inside the barn and slurry. Contaminated slurry presented the major emission source for ESBL/AmpC-producing E. coli in the pig fattening farms, but a spread via the airborne route or via different vectors also seems possible.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2014.10.010