A Single-tube real-time RT-PCR assay for RNA detection and quantification of genetically diverse HIV including rare HIV-1 non-m groups and HIV-2

The RT-PCR method with real-time fluorescence detection was used for the development of a prototype of a diagnostic kit for reliable diagnosis of genetic variants of RNA of HIV-1 groups M, N, O, and P and RNA of HIV-2 in blood plasma and serum. The kit is tolerant to nucleotide mismatches, provides...

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Bibliographic Details
Published in:Molecular genetics, microbiology and virology microbiology and virology, 2014-10, Vol.29 (4), p.179-185
Main Authors: Prasolova, M. A., Ivanov, M. K., Gashnikova, N. M., Netesova, E. S., Dymshits, G. M.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Experimental Works
Human immunodeficiency virus
Human immunodeficiency virus 1
Human immunodeficiency virus 2
Life Sciences
Microbiology
Molecular Medicine
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Summary:The RT-PCR method with real-time fluorescence detection was used for the development of a prototype of a diagnostic kit for reliable diagnosis of genetic variants of RNA of HIV-1 groups M, N, O, and P and RNA of HIV-2 in blood plasma and serum. The kit is tolerant to nucleotide mismatches, provides a broad linear range of concentrations of HIV RNA, 100% analytical specificity, and adequate analytical sensitivity: 42 ME/mL (HIV-1 group M), 45 copies/mL (HIV-2), 92 copies/mL (HIV-1 group O), and 90 copies/mL (HIV-1 group N). The kit provided successful detection and measurement of HIV RNA concentration in all the samples of the international reference panel for HIV-1 genotypes. The results of the test correlate with the results of commercial tests.
ISSN:0891-4168
1934-841X
DOI:10.3103/S0891416814040089