Lygodium flexuosum extract down regulates the expression of proinflammatory cytokines in CCl sub(4)-induced hepatotoxicity

Objective: To examine the downregulation of proinflammatory cytokines in a time dependant manner on carbon tetrachloride induced toxicity in experimental animals. Methods: CCl sub(4) (150 mu L/100 g) was dissolved in corn oil (1:1 v/v %) and administered orally. Group I was treated as normal control...

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Published in:Asian Pacific journal of tropical medicine 2012-06, Vol.5 (6), p.421-426
Main Authors: Wills, Pallara Janardhanan, Asha, Velikkakathu Vasumathi
Format: Article
Language:English
Subjects:
Lygodium
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Summary:Objective: To examine the downregulation of proinflammatory cytokines in a time dependant manner on carbon tetrachloride induced toxicity in experimental animals. Methods: CCl sub(4) (150 mu L/100 g) was dissolved in corn oil (1:1 v/v %) and administered orally. Group I was treated as normal control and received corn oil on 8th day. Group II was toxic control and was given a single dose of CCl sub(4) on 8th days. Group III was treated with Lygodium flexuosum (L. flexuosum) n-hexane extract (200 mg/kg) for 8 days and on 8th day a single dose of CCl sub(4) was received. Group IV (negative control) received L. flexuosum n-hexane extract (200 mg/kg) alone for 8 days. Results: Treatment with n-hexane extract prior to the administration of CCl sub(4) significantly prevented an increase in serum AST, ALT, LDH activity and lipid peroxidation and prevented the depletion of glutathione (GSH). Rats treated with L. flexuosum had reduced mRNA levels of TGF- beta 1, TNF- alpha and IL-1 beta genes in liver of CCl sub(4) intoxicated rats when compared to CCl sub(4) control as evidenced by RT-PCR. Conclusions: The data suggest that L. flexuosum, a widely available fern, significantly reduces CCl sub(4) induced acute hepatotoxicity by down-regulating the expression of pro-inflammatory cytokines in rats.
ISSN:1995-7645
DOI:10.1016/S1995-7645(12)60072-8