Expression of PI3-K,PKB and GSK-3β in the skeletal muscle tissue of gestational diabetes mellitus

Objective:To analyze the expression of phosphatidylinositol 3 kinase(PI3-K),protein kinase B(PKB)and glycogen synthase kinase 3 beta(GSK-3β)in skeletal muscle tissue of gestational diabetes mellitus(GDM).Methods:A total of 90 cases of pregnant women were divided into observation group and control gr...

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Published in:Asian Pacific journal of tropical medicine 2014-04, Vol.7 (4), p.309-312
Main Authors: Zhang, Tao, Fang, Min, Fu, Zi-Mu, Du, He-Chun, Yuan, Hua, Xia, Gui-Yu, Feng, Jie, Yin, Gui-Yun
Format: Article
Language:English
Subjects:
Adult
Cohort Studies
diabetes
Diabetes, Gestational - enzymology
Diabetes, Gestational - metabolism
Female
Gestational
Gestational diabetes mellitus
Glycogen
Glycogen Synthase Kinase 3 - biosynthesis
Glycogen Synthase Kinase 3 - chemistry
Glycogen Synthase Kinase 3 beta
Glycogen synthase kinase 3β
Humans
Immunohistochemistry
kinase
mellitus
Muscle, Skeletal - chemistry
Muscle, Skeletal - enzymology
Muscle, Skeletal - metabolism
musclet
Phosphatidylinositol
Phosphatidylinositol 3 kinase
Phosphatidylinositol 3-Kinase - biosynthesis
Phosphatidylinositol 3-Kinase - chemistry
Pregnancy
Protein
Protein kinase B
Proto-Oncogene Proteins c-akt - biosynthesis
Proto-Oncogene Proteins c-akt - chemistry
Skeletal
Skeletal musclet
synthase
Young Adult
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Summary:Objective:To analyze the expression of phosphatidylinositol 3 kinase(PI3-K),protein kinase B(PKB)and glycogen synthase kinase 3 beta(GSK-3β)in skeletal muscle tissue of gestational diabetes mellitus(GDM).Methods:A total of 90 cases of pregnant women were divided into observation group and control group according to the occurrence of GDM with 45 cases in either,and the expression of PI3-K,PKB,GSK-3βmRNA expression in skeletal muscle tissue was compared between two groups.Results:The total PI3-K p85 protein was significantly higher in the observation group compared with the control group,the activity of PI3-K was lower than that of the latter;The total PKB,GSK-3βprotein in skeletal tissue had no significant difference between two groups,while the serine phosphorylation levels of PKB and GSK-3βwere significantly lower in observation group compared with the control group.Conclusions:The downregulation of PI3-K,PKB and GSK-3βin skeletal tissue of GDM caused by phosphorylation dysfunction of signaling molecules is the reason for insulin resistance and transporter function decline which lead to GDM.
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(14)60045-6