Percentages of Activated and Proliferating T-Regulatory Cells Correlate with Peanut-Specific Immunoglobulin-E Level in Peanut Allergic Children

Methods Flow cytometry was used to enumerate total Treg (CD4+CD25+Foxp3+), activated Treg (CD4+CD25+Foxp3+CD45RA-) and proliferating Treg (CD4+CD25+Foxp3+CFSElo-), in resting (6-day culture in media) and crude peanut extract (CPE)-stimulated (6-day culture with CPE) peripheral blood mononuclear cell...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2015-02, Vol.135 (2), p.AB226-AB226
Main Authors: Hsiao, Kuang-Chih, MBChB, Jindal, Lalita, Ponsonby, Anne-Louise, PhD, Baker-Mackie, Monique, Lee, Cassandra, Licciardi, Paul, PhD, Tang, Mimi L.K., FRACP, PhD, FAAAAI
Format: Article
Language:English
Subjects:
Allergies
Allergy and Immunology
T cell receptors
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Summary:Methods Flow cytometry was used to enumerate total Treg (CD4+CD25+Foxp3+), activated Treg (CD4+CD25+Foxp3+CD45RA-) and proliferating Treg (CD4+CD25+Foxp3+CFSElo-), in resting (6-day culture in media) and crude peanut extract (CPE)-stimulated (6-day culture with CPE) peripheral blood mononuclear cells (PBMC) obtained from 26 peanut allergic subjects.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2014.12.1672