Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1

An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1. Microscopy data show a correlation between the intracellular levels of SOD1 and the content of zinc, corresp...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-01, Vol.51 (3), p.584-587
Main Authors: Luchinat, E, Gianoncelli, A, Mello, T, Galli, A, Banci, L
Format: Article
Language:English
Subjects:
Binding
Copper - chemistry
Fluorescence
Human
Humans
Intracellular Space
Magnetic Resonance Spectroscopy
Maturation
Microscopy
NMR spectroscopy
Nuclear magnetic resonance
Superoxide Dismutase - chemistry
Superoxide Dismutase - metabolism
Superoxide Dismutase - ultrastructure
Superoxide Dismutase-1
X-ray fluorescence
X-Rays
Zinc
Zinc - chemistry
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Summary:An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1. Microscopy data show a correlation between the intracellular levels of SOD1 and the content of zinc, corresponding to zinc binding to SOD1 observed by in-cell NMR.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc08129c