Magnetic bead and gold nanoparticle probes based immunoassay for β-casein detection in bovine milk samples

In this work, a double-probe based immunoassay was developed for rapid and sensitive determination of β-casein in bovine milk samples. In the method, magnetic beads (MBs), employed as supports for the immobilization of anti-β-casein polyclonal antibody (PAb), were used as the capture probe. Colloida...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-04, Vol.66, p.559-564
Main Authors: Li, Y.S., Meng, X.Y., Zhou, Y., Zhang, Y.Y., Meng, X.M., Yang, L., Hu, P., Lu, S.Y., Ren, H.L., Liu, Z.S., Wang, X.R.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Immobilized
Beads
Benzidines
Biosensing Techniques - methods
Biosensing Techniques - statistics & numerical data
Biosensors
Bovine milk
Bridges (structures)
Caseins - analysis
Caseins - immunology
Cattle
Gold
Horseradish Peroxidase
Immunoassay
Immunoassay - methods
Immunoassay - statistics & numerical data
Magnetic Phenomena
Metal Nanoparticles - ultrastructure
Milk
Milk - chemistry
Nanostructure
Probe
Sandwich structures
β-casein
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Summary:In this work, a double-probe based immunoassay was developed for rapid and sensitive determination of β-casein in bovine milk samples. In the method, magnetic beads (MBs), employed as supports for the immobilization of anti-β-casein polyclonal antibody (PAb), were used as the capture probe. Colloidal gold nanoparticles (AuNPs), employed as a bridge for loading anti-β-casein monoclonal antibody (McAb) and horseradish peroxidase (HRP), were used as the amplification probe. The presence of β-casein causes the sandwich structures of MBs-PAb–β-casein-McAb–AuNPs through the interaction between β-casein and the anti-β-casein antibodies. The HRP, used as an enzymatic-amplified tracer, can catalytically oxidize the substrate 3,3′,5,5′-tetramethylbenzidine (TMB), generating optical signals that are proportional to the quantity of β-casein. The linear range of the immunoassay was from 6.5 to 1520ngmL−1. The limit of detection (LOD) was 4.8ngmL−1 which was 700 times lower than that of MBs-antibody-HRP based immunoassay and 6–7 times lower than that from the microplate-antibody-HRP based assay. The recoveries of β-casein from bovine milk samples were from 95.0% to 104.3% that had a good correlation coefficient (R2=0.9956) with those obtained by an official standard Kjeldahl method. For higher sensitivity, simple sample pretreatment and shorter time requirement of the antigen–antibody reaction, the developed immunoassay demonstrated the viability for detection of β-casein in bovine milk samples. •Magnetic bead and gold nanoparticle based probes were synthesized.•The probes showed highly sensitive and selective to β-CN.•A double-probe based immunoassay for β-CN was developed.•The assay was applied to determine β-CN in bovine milk samples.•The advantages of the assay were rapidity, higher sensitivity and simple sample pretreatment.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.12.025