The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5

This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reache...

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Bibliographic Details
Published in:Brazilian Archives of Biology and Technology 2014-02, Vol.57 (1), p.45-47
Main Authors: Lima, Francisco Esmaile de Sales, Batista, Helena Beatriz Ruthner, Campos, Fabrício Souza, Kunert Filho, Hiran Castagnino, Cibulski, Samuel, Finoketti, Fernando, Arantes, Thalita, Spilki, Fernando, Roehe, Paulo Michel, Rijsewijk, Franciscus Antonius Maria, Franco, Ana Cláudia
Format: Article
Language:English
Subjects:
BoHV-5
Bovine herpesvirus 5
Parainfluenza virus
transfection
V mRNA
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Summary:This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.
ISSN:1516-8913
1678-4324
1516-8913
1678-4324
DOI:10.1590/S1516-89132014000100007