Reduction of IgE Immunoreactivity of Whole Peanut (Arachis hypogaea L.) After Pulsed Light Illumination

Pulsed light (PL), a novel food processing and preservation technology, has been shown in literature to reduce allergen levels on peanut, soybean, almond and shrimp protein extracts. This study investigated how PL affected the immunoreactivity of whole peanut kernels at two sample-to-lamp distances...

Full description

Saved in:
Bibliographic Details
Published in:Food and bioprocess technology 2014-09, Vol.7 (9), p.2637-2645
Main Authors: Zhao, Xingyu, Yang, Wade, Chung, Si-Yin, Sims, Charles A., Otwell, Steve W., Rababah, Taha M.
Format: Article
Language:English
Subjects:
Agriculture
Allergens
Arachis hypogaea
Biotechnology
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Electrophoresis
Enzyme-linked immunosorbent assay
Food processing
Food Science
Gel electrophoresis
Illumination
Immunoglobulin E
Immunoreactivity
Kernels
Light
Novel foods
Nuts
Original Paper
Peanuts
Polyacrylamide
Preservation
Proteins
Prunus dulcis
Reduction
Sodium dodecyl sulfate
Sodium lauryl sulfate
Soybeans
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pulsed light (PL), a novel food processing and preservation technology, has been shown in literature to reduce allergen levels on peanut, soybean, almond and shrimp protein extracts. This study investigated how PL affected the immunoreactivity of whole peanut kernels at two sample-to-lamp distances (7 and 10 cm) and a PL frequency of 3 pulses/s. A combination of different illumination durations and distances were tested to explore the effective conditions for PL roasting of whole peanuts. After the PL treatments, crude protein of the whole peanuts was extracted and compared to that of the raw peanuts (control) in vitro for allergen reactivity using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, dot blot, and enzyme-linked immunosorbent assay (ELISA) with pooled plasma of allergenic patients. The SDS-PAGE, Western blot, dot blot results all showed that PL was capable of mitigating all major allergens of whole peanut kernels to an undetectable level. Indirect ELISA results showed 80 % signal reduction for the PL-treated peanut as compared to raw peanut. A closer distance (7 cm) between the PL lamp and the sample resulted in stronger reduction of IgE immunoreactivity than 10 cm.
ISSN:1935-5130
1935-5149
DOI:10.1007/s11947-014-1260-7